QTLs for stomatal and photosynthetic traits related to salinity tolerance in barley

BMC Genomics

Research Article

Guang Chen¹  Gang Wang²  Sergey Shabala³  Yun Fan³  Meixue Zhou³  Mohammad Babla⁴  Zhong-Hua Chen⁴  Michelle Mak⁴  Grace Scott⁴  Paul Holford⁴  Xiaohui Liu⁵  Feifei Wang⁶ 

[1] College of Agriculture and Biotechnology, Zhejiang University, 310058, Hangzhou, China;School of Environmental Science and Engineering, Tianjin University, 300072, Tianjin, China;School of Land and Food and Tasmanian Institute of Agriculture, University of Tasmania, 7249, Hobart, TAS, Australia;School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, 2751, Penrith, NSW, Australia;School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, 2751, Penrith, NSW, Australia;School of Environmental Science and Engineering, Tianjin University, 300072, Tianjin, China;School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, 2751, Penrith, NSW, Australia;School of Land and Food and Tasmanian Institute of Agriculture, University of Tasmania, 7249, Hobart, TAS, Australia;

关键词: Gas exchange;    Guard cell;    Hordeum vulgare;    QTL mapping;    Stomatal regulation;   

DOI  :  10.1186/s12864-016-3380-0

 received in 2016-05-09, accepted in 2016-12-06,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundStomata regulate photosynthesis and transpiration, and these processes are critical for plant responses to abiotic stresses such as salinity. A barley double haploid population with 108 lines derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive) was used to detect quantitative trait loci (QTLs) associated with stomatal and photosynthetic traits related to salinity tolerance.ResultsA total of 11 significant QTLs (LOD > 3.0) and 11 tentative QTLs (2.5 < LOD < 3.0) were identified. These QTLs are distributed on all the seven chromosomes, except 5H and explain 9.5–17.3% of the phenotypic variation. QTLs for biomass, intercellular CO2 concentration, transpiration rate and stomatal conductance under control conditions co-localised together. A QTL for biomass also co-located with one for transpiration rate under salinity stress. A linkage was found between stomatal pore area and gas exchange. A QTL for salinity tolerance also co-localised with QTLs for grain yield and biomass on chromosome 3H. Based on the draft barley genome, the candidate genes for salinity tolerance at this locus are proposed.ConclusionsThe lack of major QTLs for gas exchange and stomatal traits under control and saline conditions indicates a complex relationship between salinity and leaf gas exchange due to the fact that these complex quantitative traits are under the control of multiple genes.

【 授权许可】

CC BY   
© The Author(s). 2017