Dysregulation of hepatic microRNA expression profiles with Clonorchis sinensis infection

BMC Infectious Diseases

Research Article

Xi Lu¹  Rui Chen²  Jing Shu³  Yihong Li³  Qiaoran Tang³  Xiaoli Zhang³  Su Han⁴  Jianping Cao⁵ 

[1] Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University, Harbin, China;Department of Orthopedic Surgery, The fourth Affiliated Hospital of Harbin Medical University, Harbin, China;Department of Parasitology, Harbin Medical University, 150081, Harbin, China;Department of Parasitology, Harbin Medical University, 150081, Harbin, China;National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health, MOH; National Center for International Research on Tropical Diseases; WHO Collaborating Center for Tropical Diseases, Shanghai, People’s Republic of China;National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health, MOH; National Center for International Research on Tropical Diseases; WHO Collaborating Center for Tropical Diseases, Shanghai, People’s Republic of China;

关键词: Clonorchis sinensis;    Hepatic;    MicroRNA;    Infection;   

DOI  :  10.1186/s12879-016-2058-1

 received in 2016-04-23, accepted in 2016-11-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundClonorchiasis remains an important zoonotic parasitic disease worldwide. The molecular mechanisms of host-parasite interaction are not fully understood. Non-coding microRNAs (miRNAs) are considered to be key regulators in parasitic diseases. The regulation of miRNAs and host micro-environment may be involved in clonorchiasis, and require further investigation.MethodsMiRNA microarray technology and bioinformatic analysis were used to investigate the regulatory mechanisms of host miRNA and to compare miRNA expression profiles in the liver tissues of control and Clonorchis sinensis (C. sinensis)-infected rats.ResultsA total of eight miRNAs were downregulated and two were upregulated, which showed differentially altered expression profiles in the liver tissue of C. sinensis-infected rats. Further analysis of the differentially expressed miRNAs revealed that many important signal pathways were triggered after infection with C. sinensis, which were related to clonorchiasis pathogenesis, such as cell apoptosis and inflammation, as well as genes involved in signal transduction mechanisms, such as pathways in cancer and the Wnt and Mitogen-activated protein kinases (MAPK) signaling pathways.ConclusionsThe present study revealed that the miRNA expression profiles of the host were changed by C. sinensis infection. This dysregulation in miRNA expression may contribute to the etiology and pathophysiology of clonorchiasis. These results also provide new insights into the regulatory mechanisms of miRNAs in clonorchiasis, which may present potential targets for future C. sinensis control strategies.

【 授权许可】

CC BY   
© The Author(s). 2016