Analysis of expression sequence tags from a full-length-enriched cDNA library of developing sesame seeds (Sesamum indicum)

BMC Plant Biology

Research Article

Yingzhong Zhao¹  Xuyan Dong¹  Hongyan Liu¹  Chaobo Tong¹  Hong Chen¹  Caihua Dong¹  Han Mao¹  Shengyi Liu¹  Tao Ke² 

[1] Key Laboratory for Oil Crops Biology, The Ministry of Agriculture, PR China. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No.2 Xudong Second Road, 430062, Wuhan, China;Key Laboratory for Oil Crops Biology, The Ministry of Agriculture, PR China. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No.2 Xudong Second Road, 430062, Wuhan, China;Department of Life Science and Technology, Nanyang Normal University, Wolong Road, 473061, Nanyang, China;

关键词: Gene Ontology;    Lignan;    Sesamin;    Sesamol;    Sesame Seed;   

DOI  :  10.1186/1471-2229-11-180

 received in 2011-08-20, accepted in 2011-12-24,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSesame (Sesamum indicum) is one of the most important oilseed crops with high oil contents and rich nutrient value. However, genetic improvement efforts in sesame could not get benefit from molecular biology technology due to poor DNA and RNA sequence resources. In this study, we carried out a large scale of expressed sequence tags (ESTs) sequencing from developing sesame seeds and further conducted analysis on seed storage products-related genes.ResultsA normalized and full-length enriched cDNA library from 5 ~ 30 days old immature seeds was constructed and randomly sequenced, leading to generation of 41,248 expressed sequence tags (ESTs) which then formed 4,713 contigs and 27,708 singletons with 44.9% uniESTs being putative full-length open reading frames. Approximately 26,091 of all these uniESTs have significant matches to the counterparts in Nr database of GenBank, and 21,628 of them were assigned to one or more Gene ontology (GO) terms. Homologous genes involved in oil biosynthesis were identified including some conservative transcription factors regulating oil biosynthesis such as LEAFY COTYLEDON1 (LEC1), PICKLE (PKL), WRINKLED1 (WRI1) and majority of them were found for the first time in sesame seeds. One hundred and 17 ESTs were identified possibly involved in biosynthesis of sesame lignans, sesamin and sesamolin. In total, 9,347 putative functional genes from developing seeds were identified, which accounts for one third of total genes in the sesame genome. Further analysis of the uniESTs identified 1,949 non-redundant simple sequence repeats (SSRs).ConclusionsThis study has provided an overview of genes expressed during sesame seed development. This collection of sesame full-length cDNAs covered a wide variety of genes in seeds, in particular, candidate genes involved in biosynthesis of sesame oils and lignans. These EST sequences enriched with full length will contribute to comparative genomic studies on sesame and other oilseed plants and serve as an abundant information platform for functional marker development and functional gene study.

【 授权许可】

Unknown   
© Ke et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.