Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora

BMC Microbiology

Research Article

Maristela Pereira¹  Clayton Luiz Borges¹  Patrícia Kott Tomazett¹  Célia Maria de Almeida Soares¹  Patrícia Fernanda Zambuzzi-Carvalho¹  Wellington Santos Martins²  Pedro Henrique Ferri³  Suzana Costa Santos³ 

[1] Departamento de Bioquímica e Biologia Molecular, Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, C.P. 131, 74001-970, Goiânia, GO, Brazil;Instituto de Informática, Universidade Federal de Goiás, Goiânia, Goiás, Brazil;Laboratório de Bioatividade Molecular, Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brazil;

关键词: Paracoccidioides;    Antifungal;    Oenothein B;    Transcriptome;    Cell wall;   

DOI  :  10.1186/1471-2180-13-227

 received in 2013-08-27, accepted in 2013-10-01,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe compound oenothein B (OenB), which is isolated from the leaves of Eugenia uniflora, a Brazilian Cerrado plant, interferes with Paracoccidioides yeast cell morphology and inhibits 1,3-β-D-glucan synthase (PbFKS1) transcript accumulation, which is involved in cell wall synthesis. In this work we examined the gene expression changes in Paracoccidioides yeast cells following OenB treatment in order to investigate the adaptive cellular responses to drug stress.ResultsWe constructed differential gene expression libraries using Representational Difference Analysis (RDA) of Paracoccidioides yeast cells treated with OenB for 90 and 180 min. Treatment for 90 min resulted in the identification of 463 up-regulated expressed sequences tags (ESTs) and 104 down-regulated ESTs. For the 180 min treatment 301 up-regulated ESTs and 143 down-regulated were identified. Genes involved in the cell wall biosynthesis, such as GLN1, KRE6 and FKS1, were found to be regulated by OenB. Infection experiments in macrophages corroborated the in vitro results. Fluorescence microscopy showed increased levels of chitin in cells treated with OenB. The carbohydrate polymer content of the cell wall of the fungus was also evaluated, and the results corroborated with the transcriptional data. Several other genes, such as those involved in a variety of important cellular processes (i.e., membrane maintenance, stress and virulence) were found to be up-regulated in response to OenB treatment.ConclusionsThe exposure of Paracoccidioides to OenB resulted in a complex altered gene expression profile. Some of the changes may represent specific adaptive responses to this compound in this important pathogenic fungus.

【 授权许可】

Unknown   
© Zambuzzi-Carvalho et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.