Genome-wide linkage study of atopic dermatitis in West Highland White Terriers

BMC Genetics

Research Article

Judith S Paps¹  Cary A Salzmann¹  Tonya L Harris¹  Thierry JM Olivry²  Natasha J Olby²  Dahlia M Nielsen³ 

[1] College of Veterinary Medicine, North Carolina State University, 27606, Raleigh, NC, USA;College of Veterinary Medicine, North Carolina State University, 27606, Raleigh, NC, USA;Center for Comparative Medicine and Translational Research, North Carolina State University, 27606, Raleigh, NC, USA;Department of Genetics, North Carolina State University, 27606, Raleigh, NC, USA;

关键词: Atopic Dermatitis;    House Dust Mite;    Causal Locus;    Golden Retriever;    Risk Allele Frequency;   

DOI  :  10.1186/1471-2156-12-37

 received in 2010-10-31, accepted in 2011-04-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundCanine atopic dermatitis (AD) is a common, heritable, chronic allergic skin condition prevalent in the West Highland White Terrier (WHWT). In canine AD, environmental allergens trigger an inflammatory response causing visible skin lesions and chronic pruritus that can lead to secondary bacterial and yeast infections. The disorder shares many of the clinical and histopathological characteristics of human AD and represents an animal model of this disorder that could be used to further elucidate genetic causes of human AD. Microsatellite markers genotyped in families of WHWTs affected with AD were used to perform a genome-wide linkage study in order to isolate chromosomal regions associated with the disorder.ResultsBlood samples and health questionnaires were collected from 108 WHWTs spanning three families. A linkage simulation using these 108 dogs showed high power to detect a highly penetrant mutation. Ninety WHWTs were genotyped using markers from the Minimal Screening Set 2 (MSS-2). Two hundred and fifty six markers were informative and were used for linkage analysis. Using a LOD score of 2.7 as a significance threshold, no chromosomal regions were identified with significant linkage to AD. LOD scores greater than 1.0 were located in a 56 cM region of chromosome 7.ConclusionsThe study was unable to detect any chromosomal regions significantly linked to canine AD. This could be a result of factors such as environmental modification of phenotype, incorrect assignment of phenotype, a mutation of low penetrance, or incomplete genome coverage. A genome-wide SNP association study in a larger cohort of WHWTs may prove more successful by providing higher density coverage and higher statistical power.

【 授权许可】

Unknown   
© Salzmann et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.