A novel dominant glossy mutation causes suppression of wax biosynthesis pathway and deficiency of cuticular wax in Brassica napus

BMC Plant Biology

Research Article

Lixia Zhu¹  Yanli Guo¹  Jinxiong Shen¹  Jinxing Tu¹  Chaozhi Ma¹  Bin Yi¹  Jing Wen¹  Yuanyuan Pu¹  Ping Xu¹  Tingting Liu¹  Jie Gao¹  Tingdong Fu¹  Jitao Zou² 

[1] National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, China;National Research Council Canada, S7N 0 W9, Saskatoon, Saskatchewan, Canada;

关键词: Brassica napus;    Glossy mutant;    Genetic mapping;    Wax biosynthesis;    Microarray assays;    Candidate genes;   

DOI  :  10.1186/1471-2229-13-215

 received in 2013-06-27, accepted in 2013-12-05,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe aerial parts of land plants are covered with cuticular waxes that limit non-stomatal water loss and gaseous exchange, and protect plants from ultraviolet radiation and pathogen attack. This is the first report on the characterization and genetic mapping of a novel dominant glossy mutant (BnaA.GL) in Brassica napus.ResultsTransmission electron microscopy revealed that the cuticle ultrastructure of GL mutant leaf and stem were altered dramatically compared with that of wide type (WT). Scanning electron microscopy corroborated the reduction of wax on the leaf and stem surface. A cuticular wax analysis of the GL mutant leaves further confirmed the drastic decrease in the total wax content, and a wax compositional analysis revealed an increase in aldehydes but a severe decrease in alkanes, ketones and secondary alcohols. These results suggested a likely blockage of the decarbonylation step in the wax biosynthesis pathway. Genetic mapping narrowed the location of the BnaA.GL gene to the end of A9 chromosome. A single-nucleotide polymorphism (SNP) chip assay in combination with bulk segregant analysis (BSA) also located SNPs in the same region. Two SNPs, two single sequence repeat (SSR) markers and one IP marker were located on the flanking region of the BnaA.GL gene at a distance of 0.6 cM. A gene homologous to ECERIFERUM1 (CER1) was located in the mapped region. A cDNA microarray chip assay revealed coordinated down regulation of genes encoding enzymes of the cuticular wax biosynthetic pathway in the glossy mutant, with BnCER1 being one of the most severely suppressed genes.ConclusionsOur results indicated that surface wax biosynthesis is broadly affected in the glossy mutant due to the suppression of the BnCER1 and other wax-related genes. These findings offer novel clues for elucidating the molecular basis of the glossy phenotype.

【 授权许可】

Unknown   
© Pu et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.