YKL-40 regulated epithelial-mesenchymal transition and migration/invasion enhancement in non-small cell lung cancer

BMC Cancer

Research Article

Chun-San Tai¹  Wen-Liang Chen¹  Yi-Ning Huang¹  Malvin Jefri²  Wen-Chien Huang³ 

[1] Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 1001 University Road, 300, Hsinchu, Taiwan, ROC;Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 1001 University Road, 300, Hsinchu, Taiwan, ROC;Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu, Taiwan;Institute of Traditional Medicine, National Yang Ming University, Taipei, Taiwan;Department of Thoracic Surgery, Mackay Memorial Hospital, Taipei, Taiwan;

关键词: Reverse Transcription Polymerase Chain Reaction;    NSCLC Patient;    NSCLC Cell;    NSCLC Cell Line;    NSCLC Patient Prognosis;   

DOI  :  10.1186/s12885-015-1592-3

 received in 2014-10-25, accepted in 2015-08-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundYKL-40 is a secreted inflammatory protein that its overexpression has been reported to correlate with poor outcome of various malignant diseases, especially in cancer. However, the function of this protein is still unclear.MethodsThe clinical prognosis of non-small cell lung cancers (NSCLC) patients and their clinical YKL-40 expressions were obtained from the Prognoscan database. The expressions of YKL-40 in patient samples were determined by Western Blotting assay. YKL-40 gene knockdown and overexpression were performed on NSCLC cancer cells (CL1-1 and CL1-5). The cells were investigated for their epithelial–mesenchymal transition (EMT) markers gene modulation through Western Blotting and RT-PCR. Further cell metastatic abilities were assessed by transwell migration and invasion assay.ResultIn this study, YKL-40 was observed to be highly expressed in NSCLC specimens. Furthermore, determined by the PrognoScan database analysis, patients with high expression levels of YKL-40 were found with poor prognosis. In the in vitro study, different characteristics of NSCLC cell lines (CL1-1, H23, H838, CL1-5, and H2009) were used as study models, where YKL-40 expression levels were determined to correlate with the phenotypic characteristics of cancer metastasis. In this study,YKL-40 was demonstrated to regulate EMT marker expressions such as Twist, Snail, Slug, N-cadherin, Vimentin, and E-cadherin. The protein’s affects in cancer cell migration and invasion were also observed in YKL-40 overexpression or knock down NSCLC cell lines.ConclusionAll of results from this study suggest that YKL-40 is a major factor in NSCLC metastasis. Thus, YKL-40 may serve as therapeutic targets for NSCLC patients in the future.

【 授权许可】

CC BY   
© Jefri et al. 2015