The adsorption of biomolecules to multi-walled carbon nanotubes is influenced by both pulmonary surfactant lipids and surface chemistry

Journal of Nanobiotechnology

Research

Bing Yan¹  Mathias Nelle²  Barbara Rothen-Rutishauser³  Peter Gehr³  Harald F Krug⁴  Peter Wick⁴  Michael Gasser⁵ 

[1] Department of Chemical Biology and Therapeutics, St. Jude Children's Research Hospital, 38105, Memphis, TN, USA;School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, China;Division Neonatology, Department of Paediatrics, Inselspital and University of Bern, Bern, Switzerland;Division of Histology, Institute of Anatomy, University of Bern, Bern, Switzerland;Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Materials Biology Interactions, St. Gallen, Switzerland;Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Materials Biology Interactions, St. Gallen, Switzerland;Division of Histology, Institute of Anatomy, University of Bern, Bern, Switzerland;

关键词: Surfactant;    Pulmonary Surfactant;    Neonatal Respiratory Distress Syndrome;    Surfactant Lipid;    Surfactant Component;   

DOI  :  10.1186/1477-3155-8-31

 received in 2010-11-04, accepted in 2010-12-15,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundDuring production and processing of multi-walled carbon nanotubes (MWCNTs), they may be inhaled and may enter the pulmonary circulation. It is essential that interactions with involved body fluids like the pulmonary surfactant, the blood and others are investigated, particularly as these interactions could lead to coating of the tubes and may affect their chemical and physical characteristics. The aim of this study was to characterize the possible coatings of different functionalized MWCNTs in a cell free environment.ResultsTo simulate the first contact in the lung, the tubes were coated with pulmonary surfactant and subsequently bound lipids were characterized. The further coating in the blood circulation was simulated by incubating the tubes in blood plasma. MWCNTs were amino (NH2)- and carboxyl (-COOH)-modified, in order to investigate the influence on the bound lipid and protein patterns. It was shown that surfactant lipids bind unspecifically to different functionalized MWCNTs, in contrast to the blood plasma proteins which showed characteristic binding patterns. Patterns of bound surfactant lipids were altered after a subsequent incubation in blood plasma. In addition, it was found that bound plasma protein patterns were altered when MWCNTs were previously coated with pulmonary surfactant.ConclusionsA pulmonary surfactant coating and the functionalization of MWCNTs have both the potential to alter the MWCNTs blood plasma protein coating and to determine their properties and behaviour in biological systems.

【 授权许可】

Unknown   
© Gasser et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.