Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat

BMC Plant Biology

Research Article

Paul Nicholson¹  Antoine Peraldi¹  Andrew Steed¹  Giovanni Beccari² 

[1] Department of Disease and Stress Biology, John Innes Centre, NR4 7UH, Colney Lane, Norwich, UK;Dipartimento di Scienze Agrarie e Ambientali, Facoltà di Agraria, Università degli Studi di Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy;

关键词: Fusarium;    Brachypodium distachyon;    wheat;    deoxynivalenol;    model-to-crop translation;    disease resistance;    host-pathogen interaction;   

DOI  :  10.1186/1471-2229-11-100

 received in 2011-02-04, accepted in 2011-06-03,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundFusarium species cause Fusarium head blight (FHB) and other important diseases of cereals. The causal agents produce trichothecene mycotoxins such as deoxynivalenol (DON). The dicotyledonous model species Arabidopsis thaliana has been used to study Fusarium-host interactions but it is not ideal for model-to-crop translation. Brachypodium distachyon (Bd) has been proposed as a new monocotyledonous model species for functional genomic studies in grass species. This study aims to assess the interaction between the most prevalent FHB-causing Fusarium species and Bd in order to develop and exploit Bd as a genetic model for FHB and other Fusarium diseases of wheat.ResultsThe ability of Fusarium graminearum and Fusarium culmorum to infect a range of Bd tissues was examined in various bioassays which showed that both species can infect all Bd tissues examined, including intact foliar tissues. DON accumulated in infected spike tissues at levels similar to those of infected wheat spikes. Histological studies revealed details of infection, colonisation and host response and indicate that hair cells are important sites of infection. Susceptibility to Fusarium and DON was assessed in two Bd ecotypes and revealed variation in resistance between ecotypes.ConclusionsBd exhibits characteristics of susceptibility highly similar to those of wheat, including susceptibility to spread of disease in the spikelets. Bd is the first reported plant species to allow successful infection on intact foliar tissues by FHB-causing Fusarium species. DON appears to function as a virulence factor in Bd as it does in wheat. Bd is proposed as a valuable model for undertaking studies of Fusarium head blight and other Fusarium diseases of wheat.

【 授权许可】

CC BY   
© Peraldi et al; licensee BioMed Central Ltd. 2011