Degradation of different pectins by fungi: correlations and contrasts between the pectinolytic enzyme sets identified in genomes and the growth on pectins of different origin

BMC Genomics

Research Article

Bernard Henrissat¹  Pedro M Coutinho¹  Henk A Schols²  Jan P Gerlach³  Isabelle Benoit³  Ronald P de Vries⁴ 

[1] Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, CNRS UMR 7257, Case 932, 163 Av de Luminy, 13288, Marseillecedex 9, France;Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703HD, Wageningen, The Netherlands;Microbiology & Kluyver Centre for Genomics of Industrial Fermentations, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands;Microbiology & Kluyver Centre for Genomics of Industrial Fermentations, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands;Fungal Physiology, CBS-KNAW, Uppsalalaan 8The Netherlands, 3584 CT, Utrecht;

关键词: Pectin;    Sugar Beet;    Methyl Esterification;    Patulin;    Growth Profile;   

DOI  :  10.1186/1471-2164-13-321

 received in 2012-03-19, accepted in 2012-07-07,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundPectins are diverse and very complex biomolecules and their structure depends on the plant species and tissue. It was previously shown that derivatives of pectic polymers and oligosaccharides from pectins have positive effects on human health. To obtain specific pectic oligosaccharides, highly defined enzymatic mixes are required. Filamentous fungi are specialized in plant cell wall degradation and some produce a broad range of pectinases. They may therefore shed light on the enzyme mixes needed for partial hydrolysis.ResultsThe growth profiles of 12 fungi on four pectins and four structural elements of pectins show that the presence/absence of pectinolytic genes in the fungal genome clearly correlates with their ability to degrade pectins. However, this correlation is less clear when we zoom in to the pectic structural elements.ConclusionsThis study highlights the complexity of the mechanisms involved in fungal degradation of complex carbon sources such as pectins. Mining genomes and comparative genomics are promising first steps towards the production of specific pectinolytic fractions.

【 授权许可】

CC BY   
© Benoit et al.; licensee BioMed Central Ltd. 2012