Bradykinin promotes migration and invasion of human immortalized trophoblasts

Reproductive Biology and Endocrinology

Research

Rafaela Erices¹  Francisco Lisboa¹  Jenny Corthorn²  Gloria Valdés² 

[1] Centro de Investigaciones Médicas, Escuela de Medicina Pontificia Universidad Católica, Santiago, Chile;Centro de Investigaciones Médicas, Escuela de Medicina Pontificia Universidad Católica, Santiago, Chile;Departamento de Nefrología, Escuela de Medicina Pontificia Universidad Católica, Santiago, Chile;

关键词: Endothelial Progenitor Cell;    Trophoblast Invasion;    Gelatinase Activity;    Placenta Accreta;    Cover Slide;   

DOI  :  10.1186/1477-7827-9-97

 received in 2011-03-09, accepted in 2011-07-05,  发布年份 2011

来源: Springer

【 摘 要 】

Having demonstrated that the bradykinin B2 receptor (B2R) is expressed in cells that participate in trophoblast invasion in humans and guinea-pigs, we investigated the role of bradykinin (BK) on cell migration and invasion in the HTR-8/SVneo trophoblast cell line using wound healing and invasion assays. First, we documented that HTR-8/SVneo cells expressed kallikrein, B2R, B1R, MMP-2 and MMP-9 using immunocytochemistry. Incubation with BK (10.0 microMol/L) for 18 hours increased the migration index 3-fold in comparison to controls or to cells preincubated with the B2R antagonist HOE-140. BK (10.0 microMol/L) incubation yielded a similar number of proliferating and viable cells as controls, therefore the enhanced closure of the wound cannot be attributed to proliferating cells. Incubation with BK (10.0 microMol/L) for 18 hours increased the invasion index 2-fold in comparison to controls or to cells preincubated with the antagonist of the B2R. Neither the B1R ligand Lys-des-Arg9 BK, nor its antagonist Lys-(des-Arg9-Leu8), modified migration and invasion. Further support for the stimulatory effect of B2R activation on migration and invasion is provided by the 3-fold increase in the number of filopodia per cell versus controls or cells preincubated with the B2R antagonist. Bradykinin had no effect on the cellular protein content of the B2R, nor the MMP-9 and MMP-2 gelatinase activity in the culture media varied after incubation with BK. This study adds bradykinin-acting on the B2R-to the stimuli of trophoblast migration and invasion, an effect that should be integrated to other modifications of the kallikrein-kinin system in normal and pathological pregnancies.

【 授权许可】

Unknown   
© Erices et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.