Toll-Like Receptor 6 differential expression in two pig genetic groups vaccinated against Mycoplasma hyopneumoniae

BMC Proceedings

Proceedings

Eliane Gasparino¹  Renata Veroneze²  Simone Eliza Facioni Guimarães²  Katiene Régia Silva Sousa²  Paulo Sávio Lopes²  André Mauric Frossard Ribeiro²  Paulo Roberto Nunes Goes² 

[1] Departamento de Zootecnia – Universidade Estadual de Maringá, 87020-900, MaringáParaná, Brasil;Departamento de Zootecnia – Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brasil;

关键词: Genetic Group;    TLR6 mRNA Expression;    Commercial Line;    Mycoplasma Hyopneumoniae;    SYBR Green Fluorescence;   

DOI  :  10.1186/1753-6561-5-S4-S9

来源: Springer

【 摘 要 】

BackgroundMycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia, which causes important economic losses to swine industry. The Toll-like receptors (TLRs) are pattern-recognition receptors which detect microbial presence and initiate the innate as well as the adaptative immune defense. Toll-like receptor 6 is a type I transmembrane protein that recognizes bacterial components. The aim of this study was to compare mRNA expression pattern of TLR6 gene in two genetically distinct groups of pigs vaccinated against Mycoplasma hyopneumoniae.MethodsFor each genetic group, peripheral blood was collected just before and 10 days after vaccination from 10 Naturalized Brazilian Piau breed and 10 Commercial White Line serum-negative female piglets. RNA was extracted from peripheral blood mononuclear cells (PBMCs), reverse transcripted and the qRT-PCR performed using SYBR green fluorescence system, using GAPDH gene as endogenous control. Analyses were performed by UNIVARIATE (Shapiro-Wilk test) and MIXED procedures of SAS software (version 9.0).ResultsIt was observed significant interaction between breed and vaccination, being the TLR6 mRNA expression higher in the Commercial White line than in the Piau breed after vaccination. Furthermore, there was differential expression before and after vaccination in the Commercial White line.ConclusionsAnalysis of in TLR6 gene expression showed difference between the two distinct genetic groups, however, other TLRs gene expression must be evaluated for a better understanding of innate resistance in the pig concerning Mycoplasma hyopneumoniae infection.

【 授权许可】

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© Régia Silva Sousa et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.