Monoclonal antibodies targeting the synthetic peptide corresponding to the polybasic cleavage site on H5N1 influenza hemagglutinin

Journal of Biomedical Science

Research

Li-Ann Chi¹  Alice L Yu²  Henry J Tsai³ 

[1]Department of Health and Nutrition, Asia University, Taichung, Taiwan

[2]Genomic Research Center, Academia Sinica, Taipei, Taiwan

[3]Genomic Research Center, Academia Sinica, Taipei, Taiwan

[4]Development Center for Biotechnology, Hsi-Chih City, Taiwan

[5]Department of Health and Nutrition, Asia University, Taichung, Taiwan

关键词: Monoclonal antibody;    Polybasic cleavage site;    Hemagglutinin;    H5N1 influenza;   

DOI  :  10.1186/1423-0127-19-37

 received in 2012-01-16, accepted in 2012-04-03,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAvian influenza H5N1 virus is highly pathogenic partially because its H5 hemagglutinin contains a polybasic cleavage site that can be processed by proteases in multiple organs.MethodsMonoclonal antibodies (mAb) specific to the synthetic peptide of hemagglutinin polybasic cleavage site of H5N1 virus were raised and tested for their neutralizing potential.ResultsPurified mAb showed suppression of H5N1 pseudovirus infection on Madin-Darby Canine Kidney (MDCK) cells but the efficacy was less than 50%. Since those mAb are specific to the intact uncut polybasic cleavage site of hemagglutinin, their efficacy depends on the extent of hemagglutinin cleavage on the viral surface.ConclusionsProteolytic analysis suggests the low efficacy associated with those mAb may be due to proteolytic cleavage already present on the majority of hemagglutinin prior to the infection of virus.

【 授权许可】

CC BY   
© Tsai et al; licensee BioMed Central Ltd. 2012