Folic acid-CdTe quantum dot conjugates and their applications for cancer cell targeting

Cancer Nanotechnology

Original Paper

Surya Singh¹  Wei Chen²  Preethi Suriamoorthy²  Marius Hossu²  Xing Zhang²  Guiyang Hao³  Xiankai Sun⁴  Alan G. Joly⁵ 

[1] Department of Integrative Biology, University of California, 94720, Berkeley, CA, USA;Department of Physics, The University of Texas at Arlington, 76019-0059, Arlington, TX, USA;Department of Radiology, The University of Texas Southwestern Medical Center, Dallas, TX, USA;Department of Radiology, The University of Texas Southwestern Medical Center, Dallas, TX, USA;Advanced Imaging Research Center, The University of Texas Southwestern Medical Center, Dallas, TX, USA;Pacific Northwest National Laboratory, 99352, Richland, WA, USA;

关键词: Quantum dots;    Luminescence;    Cancer;    Folic acid receptors;    Bioconjugation;    Imaging;    Labeling;    Targeting;   

DOI  :  10.1007/s12645-010-0003-3

 received in 2010-04-25, accepted in 2010-07-07,  发布年份 2010

来源: Springer

【 摘 要 】

In this study, we report the preparation, luminescence, and targeting properties of folic acid-CdTe quantum dot conjugates. Water-soluble CdTe quantum dots were synthesized and conjugated with folic acid using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide chemistry. The influence of folic acid on the luminescence properties of CdTe quantum dots was investigated, and no energy transfer between them was observed. To investigate the efficiency of folic acid-CdTe nanoconjugates for tumor targeting, pure CdTe quantum dots and folic acid-coated CdTe quantum dots were incubated with human nasopharyngeal epidermal carcinoma cell line with positive expressing folic acid receptors (KB cells) and lung cancer cells without expression of folic acid receptors (A549 cells). For the cancer cells with positive folate receptors (KB cells), the uptake for CdTe quantum dots is very low, but for folic acid-CdTe nanoconjugates, the uptake is very high. For the lung cancer cells without folate receptors (A549 cells), the uptake for folic acid-CdTe nanoconjugates is also very low. The results indicate that folic acid is an effective targeting molecule for tumor cells with overexpressed folate receptors.

【 授权许可】

Unknown   
© Springer-Verlag 2010