Eicosapentaenoic and docosahexaenoic acid supplementation and inflammatory gene expression in the duodenum of obese patients with type 2 diabetes

Nutrition Journal

Short Report

Valéry Lemelin¹  Patrick Couture²  Marie-Ève Labonté²  Jean-Charles Hogue²  Benoît Lamarche²  André J Tremblay² 

[1]Department of Gastroenterology, Laval University Hospital Research Center, 2705, boul. Laurier, G1V 4G2, Québec (Qc), Canada

[2]Institute of Nutrition and Functional Foods, Laval University, 2440 boul. Hochelaga, G1V 0A6, Québec (Qc), Canada

关键词: Eicosapentaenoic acid (EPA);    Docosahexaenoic acid (DHA);    Signal transducer and activator of transcription 3 (STAT3);    Inflammatory gene expression;    n-3 supplementation;    Placebo-controlled;    Duodenum;    Type 2 diabetes;   

DOI  :  10.1186/1475-2891-12-98

 received in 2013-02-20, accepted in 2013-07-09,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe extent to which long-chain omega-3 polyunsaturated fatty acids (LCn-3PUFA) from fish oil such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) exert their anti-inflammatory effects by down-regulating intestinal inflammation in humans is unknown. We investigated the impact of LCn-3PUFA supplementation on inflammatory gene expression in the duodenum of obese patients with type 2 diabetes.FindingsThis placebo-controlled randomized crossover study included 12 men with type 2 diabetes. After a 4-week run-in period, patients received in a random sequence 5 g/d of fish oil (providing 3 g of EPA + DHA) and a placebo (corn and soybean oil) for 8 weeks each. The two treatment phases were separated by a 12-week washout period. Gene expression was assessed by real-time polymerase chain reaction in duodenal biopsy samples obtained in the fasted state at the end of each treatment phase. Intestinal mRNA expression levels of interleukin (IL)-6 and tumor-necrosis factor (TNF)-α were hardly detectable after either treatment (<100 copies/105 copies of the reference gene ATP5o). Intestinal mRNA expression of IL-18 and of the transcription factor signal transducer and activator of transcription 3 (STAT3) was higher (>5000 copies/105 copies ATP5o) but still relatively low. EPA + DHA supplementation had no impact on any of these levels (all P ≥ 0.73).ConclusionsThese data suggest that duodenal cells gene expression of pro-inflammatory cytokines is low in patients with type 2 diabetes and not affected by EPA + DHA supplementation. Further studies are warranted to determine if inflammatory gene expression in other tissues surrounding the intestine is modulated by EPA + DHA supplementation.Trial registrationClinicalTrials.gov ID: NCT01449773

【 授权许可】

Unknown   
© Labonté et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.