Identification and independent validation of a stable yield and thousand grain weight QTL on chromosome 6A of hexaploid wheat (Triticum aestivum L.)

BMC Plant Biology

Research Article

Michelle Leverington-Waite¹  Adrian S Turner¹  Jemima Brinton¹  James Simmonds¹  Peter Scott¹  John Snape¹  Cristobal Uauy²  Viktor Korzun³ 

[1] John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK;John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK;National Institute of Agricultural Botany, Huntingdon Road, CB3 0LE, Cambridge, UK;KWS Lochow GMBH, Ferdinand-von-Lochow-Str. 5, 29303, Bergen-Wohlde, Germany;

关键词: Wheat;    Yield;    Grain size;    Grain shape;    Green canopy duration;    QTL;    NILs;   

DOI  :  10.1186/s12870-014-0191-9

 received in 2014-05-12, accepted in 2014-07-14,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundGrain yield in wheat is a polygenic trait that is influenced by environmental and genetic interactions at all stages of the plant’s growth. Yield is usually broken down into three components; number of spikes per area, grain number per spike, and grain weight (TGW). In polyploid wheat, studies have identified quantitative trait loci (QTL) which affect TGW, yet few have been validated and fine-mapped using independent germplasm, thereby having limited impact in breeding.ResultsIn this study we identified a major QTL for TGW, yield and green canopy duration on wheat chromosome 6A of the Spark x Rialto population, across 12 North European environments. Using independent germplasm in the form of BC2 and BC4 near isogenic lines (NILs), we validated the three QTL effects across environments. In four of the five experiments the Rialto 6A introgression gave significant improvements in yield (5.5%) and TGW (5.1%), with morphometric measurements showing that the increased grain weight was a result of wider grains. The extended green canopy duration associated with the high yielding/TGW Rialto allele was comprised of two independent effects; earlier flowering and delayed final maturity, and was expressed stably across the five environments. The wheat homologue (TaGW2) of a rice gene associated with increased TGW and grain width was mapped within the QTL interval. However, no polymorphisms were identified in the coding sequence between the parents.ConclusionThe discovery and validation through near-isogenic lines of robust QTL which affect yield, green canopy duration, thousand grain weight, and grain width on chromosome 6A of hexaploid wheat provide an important first step to advance our understanding of the genetic mechanisms regulating the complex processes governing grain size and yield in polyploid wheat.

【 授权许可】

Unknown   
© Simmonds et al. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.