Analysis of the humoral immune responses among cynomolgus macaque naturally infected with Reston virus during the 1996 outbreak in the Philippines

BMC Veterinary Research

Research Article

Yoshiyuki Ishii¹  Yasuhiro Yoshikawa¹  Shigeru Kyuwa¹  Tetsuro Ikegami²  Noriyo Nagata³  Hiroomi Akashi⁴  Shigeru Morikawa⁵  Shumpei Watanabe⁵  Tetsuya Mizutani⁵  Shuetsu Fukushi⁵  Masayuki Saijo⁵  Satoshi Taniguchi⁶  Yusuke Sayama⁷  Itoe Iizuka⁸  Mary E Miranda⁹ 

[1] Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo-ku, Tokyo, Japan;Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Mary Moody Northen Pavilion 3.206D, 301 University Boulevard Galveston, 77555-0436, Texas, TX, USA;Department of Pathology, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan;Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo-ku, Tokyo, Japan;Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan;Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan;Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, 113-8657, Bunkyo-ku, Tokyo, Japan;Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan;Department of Virology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Japan;Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, 208-0011, Musashimurayama, Tokyo, Japan;Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan;Veterinary Public Health Specialist, Aralia St., Ayala Westgrove Heights, Silang, 4118, Cavite, Philippines;

关键词: Ebola;    Ebolavirus;    Reston virus;    Reston ebolavirus;    Filovirus;    Zoonosis;    Humoral immune response;    Cynomolgus macaque;    Cytokine;    Antibody;   

DOI  :  10.1186/1746-6148-8-189

 received in 2012-06-15, accepted in 2012-10-04,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundEbolaviruses induce lethal viral hemorrhagic fevers (VHFs) in humans and non-human primates, with the exceptions of Reston virus (RESTV), which is not pathogenic for humans. In human VHF cases, extensive analyses of the humoral immune responses in survivors and non-survivors have shown that the IgG responses to nucleoprotein (NP) and other viral proteins are associated with asymptomatic and survival outcomes, and that the neutralizing antibody responses targeting ebolaviruses glycoprotein (GP1,2) are the major indicator of protective immunity. On the other hand, the immune responses in non-human primates, especially naturally infected ones, have not yet been elucidated in detail, and the significance of the antibody responses against NP and GP1,2 in RESTV-infected cynomolgus macaques is still unclear. In this study, we analyzed the humoral immune responses of cynomolgus macaque by using serum specimens obtained from the RESTV epizootic in 1996 in the Philippines to expand our knowledge on the immune responses in naturally RESTV-infected non-human primates.ResultsThe antibody responses were analyzed using IgG-ELISA, an indirect immunofluorescent antibody assay (IFA), and a pseudotyped VSV-based neutralizing (NT) assay. Antigen-capture (Ag)-ELISA was also performed to detect viral antigens in the serum specimens. We found that the anti-GP1,2 responses, but not the anti-NP responses, closely were correlated with the neutralization responses, as well as the clearance of viremia in the sera of the RESTV-infected cynomolgus macaques. Additionally, by analyzing the cytokine/chemokine concentrations of these serum specimens, we found high concentrations of proinflammatory cytokines/chemokines, such as IFNγ, IL8, IL-12, and MIP1α, in the convalescent phase sera.ConclusionsThese results imply that both the antibody response to GP1,2 and the proinflammatory innate responses play significant roles in the recovery from RESTV infection in cynomolgus macaques.

【 授权许可】

Unknown   
© Taniguchi et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.