Research progress of multi-enzyme complexes based on the design of scaffold protein

Bioresources and Bioprocessing

Review

Xiangyi Wang¹  Haibo Yuan¹  Hongling Liu¹  Tengfei Wang¹  Yi Jiang¹  Di Huang¹ 

[1] State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), 250353, Jinan, Shandong, People’s Republic of China;Key Laboratory of Shandong Microbial Engineering, School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), 250353, Jinan, Shandong, People’s Republic of China;

关键词: Enzyme cascades;    Substrate channel;    Scaffold protein;    Fusion protein;    Linker;   

DOI  :  10.1186/s40643-023-00695-8

 received in 2023-08-06, accepted in 2023-10-04,  发布年份 2023

来源: Springer

【 摘 要 】

Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This paper reviews the recent progress in the design strategy and application of multi-enzyme complexes. First, the metabolic channels in the multi-enzyme complex have been introduced, and the construction strategies of the multi-enzyme complex emerging in recent years have been summarized. Then, the discovered enzyme cascades related to scaffold proteins are discussed, emphasizing on the influence of the linker on the fusion enzyme (fusion protein) and its possible mechanism. This review is expected to provide a more theoretical basis for the modification of multi-enzyme complexes and broaden their applications in synthetic biology.

【 授权许可】

CC BY   
© State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology 2023

【 图 表 】

Fig. 1

Fig. 6

Fig. 2

Fig. 6