The effect of storage at ambient temperature on the feline fecal microbiota

BMC Veterinary Research

Methodology Article

Moran Tal¹  Charlotte Chau¹  Adronie Verbrugghe¹  J. Scott Weese²  Diego E. Gomez³ 

[1] Department of Clinical Studies, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada;Department of Pathobiology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada;Department of Pathobiology, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, ON, Canada;College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA;

关键词: Gastrointestinal;    Fecal;    Microbiota;    Storage;    Temperature;    Ambient;   

DOI  :  10.1186/s12917-017-1188-z

 received in 2017-03-01, accepted in 2017-08-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundFeline fecal microbiota analyses can potentially be impacted by a variety of factors such as sample preparation, sequencing method and bioinformatics analyses. Another potential influence is changes in the microbiota from storage of samples prior to processing. This study examined the effect of ambient temperature exposure on the feline fecal microbiota composition.Fecal samples were collected from 12 healthy cats, within 15 min after defecation. Samples were aliquoted and the first aliquot was frozen at −80 °C within 1 hour of defecation. Remaining aliquots were maintained at ambient temperature (20 to 23 °C) and frozen at −80 °C at 6, 12, 24, 36, 48, 72 and 96 h after collection. DNA was extracted from all aliquots, and polymerase chain reaction (PCR). The PCR products were sequenced with next-generation sequencing (Illumina MiSeq).ResultsNo significant differences were observed in alpha and beta biodiversity indexes, as well as relative abundance of different taxa over time (P > 0.05 for all tests between time points). Principal coordinate analyses demonstrated that samples cluster mainly by cat, with no significant differences between time points (AMOVA, P > 0.05; HOMOVA, P > 0.05). Linear discriminant analysis effect size method was performed and failed to detect any enriched taxa, between time points. Random forest algorithm analysis indicated homogeneity across time points.ConclusionsAlthough existing evidence from human fecal storage studies is contradictory, a recent study in companion animals agreed with the current study, demonstrating that maintenance of feline fecal samples at ambient temperature for up to 4 days has no effect on the bacterial membership and structure.

【 授权许可】

CC BY   
© The Author(s). 2017