Chemosensitization of cancer cells by siRNA using targeted nanogel delivery

BMC Cancer

Research Article

William H Blackburn¹  L Andrew Lyon¹  Michael H Smith¹  John F McDonald²  Laura B Kapa³  Erin B Dickerson⁴ 

[1] Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, 30332, Atlanta, GA, USA;Department of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, 30332, Atlanta, GA, USA;School of Biology, Georgia Institute of Technology, 310 Ferst Drive, 30332, Atlanta, GA, USA;Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, 30332, Atlanta, GA, USA;Ovarian Cancer Institute, Georgia Institute of Technology, 315 Ferst Drive, 30332, Atlanta, GA, USA;School of Biology, Georgia Institute of Technology, 310 Ferst Drive, 30332, Atlanta, GA, USA;Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, 30332, Atlanta, GA, USA;Ovarian Cancer Institute, Georgia Institute of Technology, 315 Ferst Drive, 30332, Atlanta, GA, USA;Department of Cellular and Molecular Medicine, Johns Hopkins School of Medicine, 1830 E. Monument Street, 21205, Baltimore, MD, USA;School of Biology, Georgia Institute of Technology, 310 Ferst Drive, 30332, Atlanta, GA, USA;Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, 30332, Atlanta, GA, USA;Ovarian Cancer Institute, Georgia Institute of Technology, 315 Ferst Drive, 30332, Atlanta, GA, USA;Veterinary Clinical Sciences Department, University of Minnesota, 1352 Boyd Avenue, 55108, St. Paul, MN, USA;

关键词: Epidermal Growth Factor Receptor;    Docetaxel;    Ovarian Cancer Cell;    Epidermal Growth Factor Receptor Expression;    siRNA Delivery;   

DOI  :  10.1186/1471-2407-10-10

 received in 2009-08-28, accepted in 2010-01-11,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundChemoresistance is a major obstacle in cancer treatment. Targeted therapies that enhance cancer cell sensitivity to chemotherapeutic agents have the potential to increase drug efficacy while reducing toxic effects on untargeted cells. Targeted cancer therapy by RNA interference (RNAi) is a relatively new approach that can be used to reversibly silence genes in vivo by selectively targeting genes such as the epidermal growth factor receptor (EGFR), which has been shown to increase the sensitivity of cancer cells to taxane chemotherapy. However, delivery represents the main hurdle for the broad development of RNAi therapeutics.MethodsWe report here the use of core/shell hydrogel nanoparticles (nanogels) functionalized with peptides that specially target the EphA2 receptor to deliver small interfering RNAs (siRNAs) targeting EGFR. Expression of EGFR was determined by immunoblotting, and the effect of decreased EGFR expression on chemosensitization of ovarian cancer cells after siRNA delivery was investigated.ResultsTreatment of EphA2 positive Hey cells with siRNA-loaded, peptide-targeted nanogels decreased EGFR expression levels and significantly increased the sensitivity of this cell line to docetaxel (P < 0.05). Nanogel treatment of SK-OV-3 cells, which are negative for EphA2 expression, failed to reduce EGFR levels and did not increase docetaxel sensitivity (P > 0.05).ConclusionThis study suggests that targeted delivery of siRNAs by nanogels may be a promising strategy to increase the efficacy of chemotherapy drugs for the treatment of ovarian cancer. In addition, EphA2 is a viable target for therapeutic delivery, and the siRNAs are effectively protected by the nanogel carrier, overcoming the poor stability and uptake that has hindered clinical advancement of therapeutic siRNAs.

【 授权许可】

Unknown   
© Dickerson et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.