Lipid phosphate phosphatase-3 regulates tumor growth via β-catenin and Cyclin-D1 signaling

Molecular Cancer

Research

Joseph O Humtsoe¹  Claudio Sorio²  Ishita Chatterjee³  Erin E Kohler³  Kishore K Wary³ 

[1] Department of Cell and Tissue Biology, University of California San Francisco, 521 Parnassus 18 Ave., 94143, CA, USA;Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134, Verona, Italy;Dept of Pharmacology, University of Illinois, 835 S Wolcott, Room E403, 60612, Chicago, IL, USA;

关键词: Vascular Endothelial Growth Factor;    U118 Cell;    SW480 Cell;    Sphingosine;    Athymic Nude Mouse;   

DOI  :  10.1186/1476-4598-10-51

 received in 2011-02-04, accepted in 2011-05-11,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe acquisition of proliferative and invasive phenotypes is considered a hallmark of neoplastic transformation; however, the underlying mechanisms are less well known. Lipid phosphate phosphatase-3 (LPP3) not only catalyzes the dephosphorylation of the bioactive lipid sphingosine-1-phosphate (S1P) to generate sphingosine but also may regulate embryonic development and angiogenesis via the Wnt pathway. The goal of this study was to determine the role of LPP3 in tumor cells.ResultsWe observed increased expression of LPP3 in glioblastoma primary tumors and in U87 and U118 glioblastoma cell lines. We demonstrate that LPP3-knockdown inhibited both U87 and U118 glioblastoma cell proliferation in culture and tumor growth in xenograft assays. Biochemical experiments provided evidence that LPP3-knockdown reduced β-catenin, CYCLIN-D1, and CD133 expression, with a concomitant increase in phosphorylated β-catenin. In a converse experiment, the forced expression of LPP3 in human colon tumor (SW480) cells potentiated tumor growth via increased β-catenin stability and CYCLIN-D1 synthesis. In contrast, elevated expression of LPP3 had no tumorigenic effects on primary cells.ConclusionsThese results demonstrate for the first time an unexpected role of LPP3 in regulating glioblastoma progression by amplifying β-catenin and CYCLIN-D1 activities.

【 授权许可】

Unknown   
© Chatterjee et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.