Modified protocol for in vivo imaging of wild-type mouse retina with customized miniature spectral domain optical coherence tomography (SD-OCT) device

Biological Procedures Online

Methodology

Sandeep Grover¹  Lee R Ferguson¹  Sankarathi Balaiya¹  Kakarla V Chalam¹ 

[1] Department of Ophthalmology, University of Florida College of Medicine, 580 W 8th Street, Tower 2, 3rd floor, 32209, Jacksonville, FL, USA;

关键词: Spectral domain optical coherence tomography;    Customized;    Retinal imaging;    Mouse;   

DOI  :  10.1186/1480-9222-14-9

 received in 2012-07-11, accepted in 2012-10-06,  发布年份 2012

来源: Springer

【 摘 要 】

This protocol outlines and evaluates a modified scanning procedure for a customized spectral domain optical coherence tomography (SD-OCT) imaging apparatus within the wild-type C57Bl/6 mouse posterior segment. This modified protocol allows for the capture of a 50 degree field of view spanning 3 mm by 3 mm perimeter with the optic disc as the central point. By utilizing this scanning protocol a more reliable measurement of retinal thickness can be achieved outside the fluctuating region of the optic disc. This protocol, when applied to this high resolution device, enables non-invasive in vivo histological imaging and biometric assessment of the various layers of the rodent posterior segment within a 20 – 30 min procedural time-frame. This protocol could establish a standardized method for evaluating morphological changes, with this commercial SDOCT device, when assessing longitudinal disease pathophysiology and treatment response in mouse models for future vision science research.

【 授权许可】

Unknown   
© Ferguson et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.