Singlet oxygen mediated DNA degradation by copper nanoparticles: potential towards cytotoxic effect on cancer cells

Journal of Nanobiotechnology

Short Communication

Tapas K Sengupta¹  Gregor P Jose¹  Subhankar Santra²  Swadhin K Mandal² 

[1] Department of Biological Sciences, Indian Institute of Science Education and Research-Kolkata, Mohanpur Campus, P.O. BCKV Main Office, 741252, Mohanpur, India;Department of Chemical Sciences, Indian Institute of Science Education and Research-Kolkata, Mohanpur Campus, P.O. BCKV Main Office, 741252, Mohanpur, India;

关键词: Hela Cell;    U937 Cell;    Singlet Oxygen;    Copper Nanoparticles;    Singlet Oxygen Generation;   

DOI  :  10.1186/1477-3155-9-9

 received in 2010-12-19, accepted in 2011-03-25,  发布年份 2011

来源: Springer

【 摘 要 】

The DNA degradation potential and anti-cancer activities of copper nanoparticles of 4-5 nm size are reported. A dose dependent degradation of isolated DNA molecules by copper nanoparticles through generation of singlet oxygen was observed. Singlet oxygen scavengers such as sodium azide and Tris [hydroxyl methyl] amino methane were able to prevent the DNA degradation action of copper nanoparticles confirming the involvement of activated oxygen species in the degradation process. Additionally, it was observed that the copper nanoparticles are able to exert cytotoxic effect towards U937 and Hela cells of human histiocytic lymphoma and human cervical cancer origins, respectively by inducing apoptosis. The growth characteristics of U937 and Hela cells were studied applying various concentrations of the copper nanoparticles.

【 授权许可】

Unknown   
© Jose et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.