Novel features of ARS selection in budding yeast Lachancea kluyveri

BMC Genomics

Research Article

Maki Inada¹  Ivan Liachko²  Shau Chee Claire Chung³  Katherine Cox³  Jasmine Barrow³  Eugene Cha³  Arael Seher³  Lu Yang³  Bik-Kwoon Tye³  Heather Pace³  Lindsay Hallas³  Gina Kang³  Uri Keich⁴  Emi Tanaka⁴ 

[1]Department of Biology, Ithaca College, Ithaca, NY, USA

[2]Department of Genome Sciences, University of Washington, Seattle, WA, USA

[3]Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA

[4]School of Mathematics and Statistics, University of Sydney, Sydney, Australia

关键词: Lactis;    Position Weight Matrix;    Origin Recognition Complex;    Order Markov Chain;    Approximate Confidence Interval;   

DOI  :  10.1186/1471-2164-12-633

 received in 2011-08-16, accepted in 2011-12-28,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe characterization of DNA replication origins in yeast has shed much light on the mechanisms of initiation of DNA replication. However, very little is known about the evolution of origins or the evolution of mechanisms through which origins are recognized by the initiation machinery. This lack of understanding is largely due to the vast evolutionary distances between model organisms in which origins have been examined.ResultsIn this study we have isolated and characterized autonomously replicating sequences (ARSs) in Lachancea kluyveri - a pre-whole genome duplication (WGD) budding yeast. Through a combination of experimental work and rigorous computational analysis, we show that L. kluyveri ARSs require a sequence that is similar but much longer than the ARS Consensus Sequence well defined in Saccharomyces cerevisiae. Moreover, compared with S. cerevisiae and K. lactis, the replication licensing machinery in L. kluyveri seems more tolerant to variations in the ARS sequence composition. It is able to initiate replication from almost all S. cerevisiae ARSs tested and most Kluyveromyces lactis ARSs. In contrast, only about half of the L. kluyveri ARSs function in S. cerevisiae and less than 10% function in K. lactis.ConclusionsOur findings demonstrate a replication initiation system with novel features and underscore the functional diversity within the budding yeasts. Furthermore, we have developed new approaches for analyzing biologically functional DNA sequences with ill-defined motifs.

【 授权许可】

Unknown   
© Liachko et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.