Far infra-red therapy promotes ischemia-induced angiogenesis in diabetic mice and restores high glucose-suppressed endothelial progenitor cell functions

Cardiovascular Diabetology

Original Investigation

Chih-Pei Lin¹  Ying-Hwa Chen²  Jaw-Wen Chen³  Shing-Jong Lin⁴  Hsin-Bang Leu⁵  Po-Hsun Huang⁶  Chao-Hung Wang⁷ 

[1] Department of Pathology and Laboratory Medicine, Division of General Laboratory, Taipei Veterans General Hospital, Taipei, Taiwan;Department of Biotechnology and Laboratory Science in Medicine and Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, Taiwan;Division of Cardiology, Taipei Veterans General Hospital, Taipei, Taiwan;Cardiovascular Research Center, National Yang-Ming University, Taipei, Taiwan;Division of Cardiology, Taipei Veterans General Hospital, Taipei, Taiwan;Department of Medical Research and Education, Taipei Veterans General Hospital, No. 201, Sec. 2, Shih-Pai Road, Taipei, Taiwan;Cardiovascular Research Center, National Yang-Ming University, Taipei, Taiwan;Institute and Department of Pharmacology, National Yang-Ming University, Taipei, Taiwan;Division of Cardiology, Taipei Veterans General Hospital, Taipei, Taiwan;Department of Medical Research and Education, Taipei Veterans General Hospital, No. 201, Sec. 2, Shih-Pai Road, Taipei, Taiwan;Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan;Cardiovascular Research Center, National Yang-Ming University, Taipei, Taiwan;Division of Cardiology, Taipei Veterans General Hospital, Taipei, Taiwan;Healthcare and Management Center, Taipei Veterans General Hospital, Taipei, Taiwan;Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan;Cardiovascular Research Center, National Yang-Ming University, Taipei, Taiwan;Division of Cardiology, Taipei Veterans General Hospital, Taipei, Taiwan;Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan;Cardiovascular Research Center, National Yang-Ming University, Taipei, Taiwan;Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan;Division of Cardiology, Chang Gung Memorial Hospital, Keelung, and Chang Gung University College of Medicine, Taoyuan, Taiwan;

关键词: Far infra-red therapy;    Endothelial progenitor cell;    Diabetes;    Ischemia;   

DOI  :  10.1186/1475-2840-11-99

 received in 2012-07-03, accepted in 2012-08-08,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundFar infra-red (IFR) therapy was shown to exert beneficial effects in cardiovascular system, but effects of IFR on endothelial progenitor cell (EPC) and EPC-related vasculogenesis remain unclear. We hypothesized that IFR radiation can restore blood flow recovery in ischemic hindlimb in diabetic mice by enhancement of EPCs functions and homing process.Materials and methodsStarting at 4 weeks after the onset of diabetes, unilateral hindlimb ischemia was induced in streptozotocine (STZ)-induced diabetic mice, which were divided into control and IFR therapy groups (n = 6 per group). The latter mice were placed in an IFR dry sauna at 34°C for 30 min once per day for 5 weeks.ResultsDoppler perfusion imaging demonstrated that the ischemic limb/normal side blood perfusion ratio in the thermal therapy group was significantly increased beyond that in controls, and significantly greater capillary density was seen in the IFR therapy group. Flow cytometry analysis showed impaired EPCs (Sca-1+/Flk-1+) mobilization after ischemia surgery in diabetic mice with or without IFR therapy (n = 6 per group). However, as compared to those in the control group, bone marrow-derived EPCs differentiated into endothelial cells defined as GFP+/CD31+ double-positive cells were significantly increased in ischemic tissue around the vessels in diabetic mice that received IFR radiation. In in-vitro studies, cultured EPCs treated with IFR radiation markedly augmented high glucose-impaired EPC functions, inhibited high glucose-induced EPC senescence and reduced H2O2 production. Nude mice received human EPCs treated with IFR in high glucose medium showed a significant improvement in blood flow recovery in ischemic limb compared to those without IFR therapy. IFR therapy promoted blood flow recovery and new vessel formation in STZ-induced diabetic mice.ConclusionsAdministration of IFR therapy promoted collateral flow recovery and new vessel formation in STZ-induced diabetic mice, and these beneficial effects may derive from enhancement of EPC functions and homing process.

【 授权许可】

Unknown   
© Huang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.