Prevalence of K13-propeller polymorphisms in Plasmodium falciparum from China-Myanmar border in 2007–2012

Malaria Journal

Research

Liwang Cui¹  Zenglei Wang¹  Mynthia Cabrera¹  Sony Shrestha¹  Jun Miao¹  Xiaolian Li¹  Caitlin Grube¹  Lili Yuan²  Zhaoqing Yang³ 

[1] Department of Entomology, The Pennsylvania State University, 16802, University Park, PA, USA;Department of Entomology, The Pennsylvania State University, 16802, University Park, PA, USA;Department of Pathogen Biology and Immunology, Kunming Medical University, 650500, Kunming, Yunnan Province, China;Department of Pathogen Biology and Immunology, Kunming Medical University, 650500, Kunming, Yunnan Province, China;

关键词: Malaria;    Plasmodium falciparum;    artemisinin resistance;    K13;    F3D7_1343700;   

DOI  :  10.1186/s12936-015-0672-9

 received in 2015-01-23, accepted in 2015-03-16,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe recent emergence and spread of artemisinin resistance in the Greater Mekong Subregion poses a great threat to malaria control and elimination. A K13-propeller gene (K13), PF3D7_1343700, has been associated lately with artemisinin resistance both in vitro and in vivo. This study aimed to investigate the K13 polymorphisms in Plasmodium falciparum parasites from the China-Myanmar border area where artemisinin use has the longest history.MethodsA total of 180 archived P. falciparum isolates containing 191 parasite clones, mainly collected in 2007–2012 from the China-Myanmar area, were used to obtain the full-length K13 gene sequences.ResultsSeventeen point mutations were identified in 46.1% (88/191) parasite clones, of which seven were new. The F446I mutation predominated in 27.2% of the parasite clones. The C580Y mutation that is correlated with artemisinin resistance was detected at a low frequency of 1.6%. Collectively, 43.1% of the parasite clones contained point mutations in the kelch domain of the K13 gene. Moreover, there was a trend of increase in the frequency of parasites carrying kelch domain mutations through the years of sample collection. In addition, a microsatellite variation in the N-terminus of the K13 protein was found to have reached a high frequency (69.1%).ConclusionsThis study documented the presence of mutations in the K13 gene in parasite populations from the China-Myanmar border. Mutations present in the kelch domain have become prevalent (>40%). A predominant mutation F446I and a prevalent microsatellite variation in the N-terminus were identified, but their importance in artemisinin resistance remains to be elucidated.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.