Effect of HXT 1 and HXT 7 hexose transporter overexpression on wild-type and lactic acid producing Saccharomyces cerevisiae cells

Microbial Cell Factories

Research

Paola Branduardi¹  Danilo Porro¹  Giorgia Rossi¹  Michael Sauer² 

[1] Department of Biotechnology and Bioscience, Università degli Studi di Milano-Bicocca, P.zza della Scienza 2, 20126, Milano, Italy;School of Bioengineering, FH Campus Wien, University of Applied Sciences, Muthgasse 18, 1190, Vienna, Austria;Department of Biotechnology, BOKU, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190, Vienna, Austria;

关键词: Lactic Acid;    Yeast Strain;    Glucose Consumption;    Lactic Acid Production;    Glycolytic Flux;   

DOI  :  10.1186/1475-2859-9-15

 received in 2009-10-13, accepted in 2010-03-09,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSince about three decades, Saccharomyces cerevisiae can be engineered to efficiently produce proteins and metabolites. Even recognizing that in baker's yeast one determining step for the glucose consumption rate is the sugar uptake, this fact has never been conceived to improve the metabolite(s) productivity.In this work we compared the ethanol and/or the lactic acid production from wild type and metabolically engineeredS. cerevisiae cells expressing an additional copy of one hexose transporter.ResultsDifferent S. cerevisiae strains (wild type and metabolically engineered for lactic acid production) were transformed with the HXT 1 or the HXT 7 gene encoding for hexose transporters.Data obtained suggest that the overexpression of an Hxt transporter may lead to an increase in glucose uptake that could result in an increased ethanol and/or lactic acid productivities. As a consequence of the increased productivity and of the reduced process timing, a higher production was measured.ConclusionMetabolic pathway manipulation for improving the properties and the productivity of microorganisms is a well established concept. A high production relies on a multi-factorial system. We showed that by modulating the first step of the pathway leading to lactic acid accumulation an improvement of about 15% in lactic acid production can be obtained in a yeast strain already developed for industrial application.

【 授权许可】

Unknown   
© Rossi et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.