Large-scale microsatellite development in grasspea (Lathyrus sativus L.), an orphan legume of the arid areas

BMC Plant Biology

Research Article

Robert Redden¹  Marina Burlyaeva²  Shiv Kumar³  Xiaopeng Hao⁴  Jianwu Chang⁴  Xuelian Sun⁵  Fang Wang⁵  Junye Jiang⁵  Jianping Guan⁵  Tao Yang⁵  Xuxiao Zong⁵  Clarice J Coyne⁶  Jinguo Hu⁶ 

[1] Australian Temperate Field Crops Collection, Grains Innovation Park, The Department of Primary Industries, Private Bag 260, 3401, Horsham, Victoria, Australia;Department of Leguminous Crops Genetic Resources, N. I. Vavilov Research Institute of Plant Industry, 190000, St. Petersburg, Russia;International Center for Agricultural Research in the Dry Areas (ICARDA), 5466, Aleppo, Syria;The Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of Ministry of Agriculture/Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences, 030031, Taiyuan, China;The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081, Beijing, China;USDA-ARS Western Regional Plant Introduction Station (WRPIS), 99164, Pullman, WA, USA;

关键词: Lathyrus sativus;    Microsatellite;    454 FLX Titanium pyrosequencing;    Marker development;   

DOI  :  10.1186/1471-2229-14-65

 received in 2013-07-19, accepted in 2014-03-12,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundGrasspea (Lathyrus sativus L., 2n = 14), a member of the family Leguminosae, holds great agronomic potential as grain and forage legume crop in the arid areas for its superb resilience to abiotic stresses such as drought, flood and salinity. The crop could not make much progress through conventional breeding in the past, and there are hardly any detailed molecular biology studies due to paucity of reliable molecular markers representative of the entire genome.ResultsUsing the 454 FLX Titanium pyrosequencing technique, 651,827 simple sequence repeat (SSR) loci were identified and 50,144 nonredundant primer pairs were successfully designed, of which 288 were randomly selected for validation among 23 L. sativus and one L. cicera accessions of diverse provenance. 74 were polymorphic, 70 monomorphic, and 144 with no PCR product. The number of observed alleles ranged from two to five, the observed heterozygosity from 0 to 0.9545, and Shannon’s information index ranged from 0.1013 to 1.0980, respectively. The dendrogram constructed by using unweighted pair group method with arithmetic mean (UPGMA) based on Nei's genetic distance, showed obvious distinctions and understandable relationships among the 24 accessions.ConclusionsThe large number of SSR primer pairs developed in this study would make a significant contribution to genomics enabled improvement of grasspea.

【 授权许可】

Unknown   
© Yang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.