A pectin-honey hydrogel prevents postoperative intraperitoneal adhesions in a rat model

BMC Veterinary Research

Research Article

Selina Iussich¹  Emanuela Morello¹  Marco Gandini¹  Gessica Giusto¹  Rosangela Odore¹  Cristina Vercelli¹  Andrea Audisio¹ 

[1] Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini, n. 2, Grugliasco, 10095, Turin, Italy;

关键词: Honey;    Pectin;    Hydrogel;    Adhesion;    Prevention;    Rats;   

DOI  :  10.1186/s12917-017-0965-z

 received in 2016-04-27, accepted in 2017-02-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAdhesions are a common postoperative surgical complication. Liquid honey has been used intraperitoneally to reduce the incidence of these adhesions. However, solid barriers are considered more effective than liquids in decreasing postoperative intra-abdominal adhesion formation; therefore, a new pectin-honey hydrogel (PHH) was produced and its effectiveness was evaluated in a rat cecal abrasion model.Standardized cecal/peritoneal abrasion was performed through laparotomy in 48 adult Sprague-Dawley rats to induce peritoneal adhesion formation. Rats were randomly assigned to a control (C) and treatment (T) group. In group T, PHHs were placed between the injured peritoneum and cecum. Animals were euthanized on day 15 after surgery. Adhesions were evaluated macroscopically and adhesion scores were recorded and compared between the two groups. Inflammation, fibrosis, and neovascularization were histologically graded and compared between the groups.ResultsIn group C, 17 of 24 (70.8%) animals developed adhesions between the cecum and peritoneum, while in group T only 5 of 24 (20.8%) did (p = 0.0012). In group C, one rat had an adhesion score of 3, sixteen had scores of 2, and seven rats had scores of 0. In group T, four rats had adhesion scores of 2, one rat had an adhesion score of 1 and nineteen have score 0 (p = 0.0003). Significantly lower grades of inflammation, fibrosis, and neovascularization were seen in group T (p = 0.006, p = 0.001, p = 0.002, respectively).ConclusionPHH is a novel absorbable barrier that is effective in preventing intra-abdominal adhesions in a cecal abrasion model in rats.

【 授权许可】

CC BY   
© The Author(s). 2017