EcoTILLING in Beta vulgaris reveals polymorphisms in the FLC-like gene BvFL1that are associated with annuality and winter hardiness

BMC Plant Biology

Research Article

Christian Jung¹  Friedrich J Kopisch-Obuch¹  Sebastian LM Frerichmann¹  Martin Kirchhoff²  Andreas E Müller³  Axel J Scheidig⁴ 

[1] Plant Breeding Institute, Christian-Albrechts-University of Kiel, 24098, Olshausenstr. 40, Kiel, Germany;Plant Breeding Institute, Christian-Albrechts-University of Kiel, 24098, Olshausenstr. 40, Kiel, Germany;Nordsaat Saatzucht GmbH, Böhnshauser Straße, 38895, Langenstein, Germany;Plant Breeding Institute, Christian-Albrechts-University of Kiel, 24098, Olshausenstr. 40, Kiel, Germany;Strube Research GmbH & Co. KG, Hauptstr. 1, 38387, Söllingen, Germany;Zoological Institute, Department of Structural Biology, Christian-Albrechts-University of Kiel, Am Botanischen Garten 1-9, 24118, Kiel, Germany;

关键词: Beta vulgaris;    EcoTILLING;    Diversity;    Flowering;    Winter hardiness;    BvFL1;   

DOI  :  10.1186/1471-2229-13-52

 received in 2012-09-12, accepted in 2013-02-21,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundSugar beet (Beta vulgaris ssp. vulgaris L.) is an important crop for sugar and biomass production in temperate climate regions. Currently sugar beets are sown in spring and harvested in autumn. Autumn-sown sugar beets that are grown for a full year have been regarded as a cropping system to increase the productivity of sugar beet cultivation. However, for the development of these “winter beets” sufficient winter hardiness and a system for bolting control is needed. Both require a thorough understanding of the underlying genetics and its natural variation.ResultsWe screened a diversity panel of 268 B. vulgaris accessions for three flowering time genes via EcoTILLING. This panel had been tested in the field for bolting behaviour and winter hardiness. EcoTILLING identified 20 silent SNPs and one non-synonymous SNP within the genes BTC1, BvFL1 and BvFT1, resulting in 55 haplotypes. Further, we detected associations of nucleotide polymorphisms in BvFL1 with bolting before winter as well as winter hardiness.ConclusionsThese data provide the first genetic indication for the function of the FLC homolog BvFL1 in beet. Further, it demonstrates for the first time that EcoTILLING is a powerful method for exploring genetic diversity and allele mining in B. vulgaris.

【 授权许可】

Unknown   
© Frerichmann et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.