期刊论文详细信息
BMC Genomics
The direction of cross affects obesity after puberty in male but not female offspring
Research Article
Danny Arends1  Jan Trost1  Gudrun A. Brockmann1  Stefan Kärst1  Sebastian Heise1  Marie-Laure Yaspo2  Vyacheslav Amstislavskiy2  Hans Lehrach2  Thomas Risch2 
[1] Albrecht Daniel Thaer-Institut für Agrar- und Gartenbauwissenschaften, Humboldt-Universität zu Berlin, Invalidenstraße 42, D-10115, Berlin, Germany;Max Planck Institute for Molecular Genetics, Gene Regulation and Systems Biology of Cancer, Ihnestraße 63-73, 14195, Berlin, Germany;
关键词: Allele-specific gene expression;    Allelic imbalance;    High fat diet;    Imprinting;    Peg3;    Circadian rhythm;    Sex differences;    Sex hormones;   
DOI  :  10.1186/s12864-015-2164-2
 received in 2015-05-19, accepted in 2015-10-29,  发布年份 2015
来源: Springer
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【 摘 要 】

BackgroundWe investigated parent-of-origin and allele-specific expression effects on obesity and hepatic gene expression in reciprocal crosses between the Berlin Fat Mouse Inbred line (BFMI) and C57Bl/6NCrl (B6N).ResultsWe found that F1-males with a BFMI mother developed 1.8 times more fat mass on a high fat diet at 10 weeks than F1-males of a BFMI father. The phenotype was detectable from six weeks on and was preserved after cross-fostering. RNA-seq data of liver provided evidence for higher biosynthesis and elongation of fatty acids (p = 0.00635) in obese male offspring of a BFMI mother versus lean offspring of a BFMI father. Furthermore, fatty acid degradation (p = 0.00198) and the peroxisome pathway were impaired (p = 0.00094). The circadian rhythm was affected as well (p = 0.00087). Among the highest up-regulated protein coding genes in obese males were Acot4 (1.82 fold, p = 0.022), Cyp4a10 (1.35 fold, p = 0.026) and Cyp4a14 (1.32 fold, p = 0.012), which hydroxylize fatty acids and which are known to be increased in liver steatosis. Obese males showed lower expression of the genetically imprinted and paternally expressed 3 (Peg3) gene (0.31 fold, p = 0.046) and higher expression of the androgen receptor (Ar) gene (2.38 fold, p = 0.068). Allelic imbalance was found for expression of ATP-binding cassette transporter gene Abca8b. Several of the differentially expressed genes contain estrogen response elements.ConclusionsParent-of-origin effects during gametogenesis and/or fetal development in an obese mother epigenetically modify the transcription of genes that lead to enhanced fatty acid synthesis and impair β-oxidation in the liver of male, but not female F1 offspring. Down-regulation of Peg3 could contribute to trigger this metabolic setting. At puberty, higher amounts of the androgen receptor and altered access to estrogen response elements in affected genes are likely responsible for male specific expression of genes that were epigenetically triggered. A suggestive lack of estrogen binding motifs was found for highly down-regulated genes in adult hepatocytes of obese F1 males (p = 0.074).

【 授权许可】

CC BY   
© Kärst et al. 2015

【 预 览 】
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Fig. 2

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