Immunization with Streptococcus suis bacterin plus recombinant Sao protein in sows conveys passive immunity to their piglets

BMC Veterinary Research

Research Article

Jai-Wei Lee¹  Wen-Bin Chung²  Kai-Jen Hsueh³  Chun-Yen Chu⁴  Yao-Chi Chung⁴  Li-Ting Cheng⁴ 

[1] Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, 91201, Pingtung, Taiwan;Department of Veterinary medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, 91201, Pingtung, Taiwan;Department of Veterinary medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, 91201, Pingtung, Taiwan;Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, 1, Shuehfu Road, Neipu, 91201, Pingtung, Taiwan;Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, 1, Shuehfu Road, Neipu, 91201, Pingtung, Taiwan;

关键词: Streptococcus suis;    rSao;    Vaccine;    Passive immunity;    Cytokine;   

DOI  :  10.1186/s12917-016-0937-8

 received in 2015-07-28, accepted in 2016-12-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundStreptococcus suis (S. suis) causes arthritis, meningitis, septicemia, and sudden death in pigs and is also an zoonotic agent for humans. The present study demonstrated that immunization with recombinant Sao-L (surface antigen one-L, rSao-L) protein from a strain of S. suis serotype 2 in pigs was able to increase cross-serotype protection against S. suis serotype 1 and 2 challenge. Since weaning piglets are more susceptible to S. suis infections due to the stresses associated with weaning, prepartum immunization in sows may convey passive immunity to piglets and provide protection.ResultsPregnant sows were immunized with a vaccine containing inactivated S. suis serotype 2 plus rSao as the antigens. Blood samples were collected from their piglets after birth for analysis of antigen-specific antibody titers and levels of various cytokines. Results demonstrated that the titers of S. suis and rSao-specific antibodies were significantly (p < 0.05) higher in the vaccinated piglets in comparison with that of piglets in the control group. The serum levels of interferon (IFN)-γ, interleukin (IL)-4, IL-6, and IL-12 were significantly (p < 0.05) increased in piglets born from vaccinated sows when compared to piglets from unvaccinated sows. In addition, piglets were challenged by heterologous and homologous S. suis. All piglets from unvaccinated sows developed severe symptoms of bacteremia, fever, anorexia, depression, and arthritis. On the other hand, piglets from vaccinated sows had significantly (p < 0.05) reduced clinical symptoms and lesion score (by 75 and 81%).ConclusionsOur results revealed that immunizing pregnant sows with the vaccine containing inactivated S. suis bacterin plus rSao as the antigens is able to enhance passive immunity against heterologous and homologous S. suis challenge in their piglets.

【 授权许可】

CC BY   
© The Author(s). 2017