Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments

BMC Bioinformatics

Software

Victor Dillard¹  Soren H. Hough¹  Kris Kancleris¹  Keith Dunaway¹  Leigh Brody¹  Neil Humphryes-Kirilov¹  Joseph Wolanski¹  Ayokunmi Ajetunmobi¹ 

[1] Desktop Genetics, Ltd., 28 Hanbury Street, E1 6QR, London, UK;

关键词: CRISPR;    sgRNA;    RNA guide design software;    Cas9;    Genome editing;    Functional genomics;   

DOI  :  10.1186/s12859-017-1581-4

 received in 2016-11-22, accepted in 2017-03-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundGuide Picker (https://www.deskgen.com/guide-picker/) serves as a meta tool for designing CRISPR experiments by presenting ten different guide RNA scoring functions in one simple graphical interface. It allows investigators to simultaneously visualize and sort through every guide targeting the protein-coding regions of any mouse or human gene.ResultsUtilizing a multidimensional graphical display featuring two plots and four axes, Guide Picker can analyze all guides while filtering based on four different criteria at a time. Guide Picker further facilitates the CRISPR design process by using pre-computed scores for all guides, thereby offering rapid guide RNA generation and selection.ConclusionsThe ease-of-use of Guide Picker complements CRISPR itself, matching a powerful and modular biological system with a flexible online web tool that can be used in a variety of genome editing experimental contexts.

【 授权许可】

CC BY   
© The Author(s). 2017