Right atrial pressure alterations during echocardiography-guided-catheterization predict tricuspid valvular impairment: a novel method for the creation of a rabbit model of Staphylococcus aureus endocarditis

Cardiovascular Ultrasound

Research

En-jie Luo¹  Mei-lian Wang¹  Miao Fan²  Ya-jun Guo³  Ying Zhang³  Wei-dong Ren³ 

[1] Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University, No. 92 Beier Road, 110001, Shenyang, Heping District, China;Department of Radiology, The First Affiliated Hospital of Sun Yat-sen University, 510080, Guangzhou, China;Department of Sonography, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, 110004, Shenyang, Heping District, China;

关键词: Infective endocarditis;    Echocardiography;    Catheterization;    Right atrial pressure;    Animal model;   

DOI  :  10.1186/1476-7120-12-21

 received in 2014-05-08, accepted in 2014-06-18,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundWe previously reported the use of a catheter system to damage the tricuspid valve and create infectious endocarditis (IE) in an animal model. The current study aims to create a faint IE model suitable for antibiotic prophylaxis using a low bacterial inoculum. We also aim to explore a way to quantitatively assess valvular impairment and to predict the success of the IE models during catheterization.MethodsNinety rabbits were assigned to two groups according to the density of bacteria inoculated (1 × 105 CFU for Group A and 1 × 104 CFU for Group B). A catheter system consisting of a polyethylene catheter and a guide wire were used to damage the valve. The catheter system was passed through the rabbits’ tricuspid valves under echocardiographic guidance. A pressure transducer was used to assess right atrial pressure (PRA) before and just after valvular damage to calculate the pressure alterations (ΔPRA). The animals in group A and B were divided into 3 subgroups according to the ΔPRA (0–5 mmHg for Groups A1 and B1; 5–10 mmHg for Groups A2 and B2; 10–15 mmHg for Groups A3 and B3). Staphylococcus aureus (ATCC 29213) inoculation was performed 24 hr after cardiac catheterization.ResultsFaint IE was confirmed in 20%, 93.3%, 26.7%, 6.7%, 20%, and 33.3% of the rabbits in Groups A1, A2, A3, B1, B2, and B3, respectively. There was no difference in the LV/RV ratio and VTR of the No-IE, faint-IE, and severe IE animals. Faint IE rabbits had a larger ΔPRA than No-IE rabbits (7.81 ± 1.21 vs. 2.48 ± 1.0, P < 0.01, for Group A; 7.60 ± 1.32 vs. 2.98 ± 1.08, P < 0.01, for Group B). The ΔPRA of severe IE and faint IE rabbits was significantly different (13.11 ± 1.31 vs. 7.81 ± 1.21, P < 0.01, for Group A; 12.73 ± 1.44 vs. 7.60 ± 1.32, P < 0.01, for Group B).ConclusionΔPRA could be used to assess valvular impairment. Controlling the value of ΔPRA during catheterization and inoculating of an appropriate dose of bacteria was associated with a successful IE model.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.