Human giant congenital melanocytic nevus exhibits potential proteomic alterations leading to melanotumorigenesis

Proteome Science

Research

Yong Kyu Kim¹  Kwan Chul Tark²  Chul Gyoo Park³  Je-Yoel Cho⁴  Hyoung Kyu Kim⁵  Seung Hun Jeong⁵  Nari Kim⁵  Kyoung Soo Ko⁵  Byoung Doo Rhee⁵  In-Sung Song⁵  Sung-Ryul Lee⁵  Min Hee Kim⁵  Dae Yun Seo⁵  Jin Han⁵ 

[1] Apgujung YK Plastic Surgery Center, Seoul, Korea;Department of Plastic and Reconstructive Surgery, College of Medicine, Yonsei University, Seoul, Korea;Department of Plastic and Reconstructive Surgery, National Medical Center, Seoul, Korea;Department of Veterinary Biochemistry, College of Veterinary Medicine, Seoul National University, 151-742, Seoul, Korea;National Research Laboratory for Mitochondrial Signaling, Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University, Busan, Korea;

关键词: Giant congenital melanocytic nevi;    Melanotumorigenesis;    Proteomics;    14-3-3 epsilon;    14-3-3 tau;    Systemic analysis;   

DOI  :  10.1186/1477-5956-10-50

 received in 2012-04-09, accepted in 2012-07-26,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundA giant congenital melanocytic nevus (GCMN) is a malformation of the pigment cells. It is a distress to the patients for two reasons: one is disfigurement, and the other is the possibility of malignant changes. However, the underlying mechanisms of the development of GCMN and melanotumorigenesis in GCMN are unknown. Hence, the aim of this study was to identify the proteomic alterations and associated functional pathways in GCMN.ResultsProteomic differences between GCMN (n = 3) and normal skin samples (n = 3) were analyzed by one-dimensional-liquid chromatography-tandem mass spectrometry Relative levels of the selected proteins were validated using western blot analysis. The biological processes associated with the abundance modified proteins were analyzed using bioinformatic tools. Among the 46 abundance modified proteins, expression of 4 proteins was significantly downregulated and expression of 42 proteins was significantly upregulated in GCMN compared to normal skin samples (p < 0.05). More importantly, 31% of the upregulated proteins were implicated in various cancers, with five proteins being specifically related with melanoma. The abundance modified proteins in GCMN were involved in the biological processes of neurotrophin signaling, melanosome, and downregulated of MTA-3 in ER-negative breast tumors. In particular, an increase in the expression of the 14-3-3 protein family members appeared to be associated with key cellular biological functions in GCMN. Western blot analysis confirmed the upregulation of 14-3-3epsilon, 14-3-3 tau, and prohibitin in GCMN.ConclusionThese findings suggest that GCMN exhibits potential proteomic alterations, which may play a role in melanotumorigenesis, and the significant alteration of 14-3-3 family proteins could be a key regulator of the biological pathway remodeling in GCMN.

【 授权许可】

Unknown   
© Kim et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.