Deficiency of innate-like T lymphocytes in chronic obstructive pulmonary disease

Respiratory Research

Research

Nelli Farkas¹  Péter Engelmann²  Kornélia Bodó²  Tímea Berki²  Mariann Szabó³  Zoltán Balikó³  Veronika Sárosi³ 

[1] Department of Bioanalysis, Medical School, University of Pécs, Szigeti u. 12, H-7643, Pécs, Hungary;Department of Immunology and Biotechnology, Clinical Center, University of Pécs, Szigeti u. 12, H-7643, Pécs, Hungary;Division of Pulmonology, 1st Department of Internal Medicine, Clinical Center, University of Pécs, Rákóczi u. 2, H-7623, Pécs, Hungary;

关键词: COPD;    Emphysema;    iNKT cells;    MAIT cells;    CD1d;    MR1;   

DOI  :  10.1186/s12931-017-0671-1

 received in 2017-07-10, accepted in 2017-10-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundBased on the phenotypic and functional characteristics unconventional T-lymphocytes such as invariant natural killer T (iNKT) cells and mucosal-associated invariant T (MAIT) cells link the innate and adaptive immune responses. Up to now data are scarce about their involvement in pulmonary disorders including chronic obstructive pulmonary disease (COPD). This study explores simultaneously the frequencies of iNKT and MAIT cells in the peripheral blood and sputum of stable and exacerbating COPD patients.MethodsBy means of multicolor flow cytometry frequencies of total iNKT and MAIT cells and their subsets were enumerated in peripheral blood and sputum samples of healthy controls, and COPD patients. In addition, gene expression of TCR for iNKT, MAIT cells, and CD1d, MR1 were assessed by qPCR in the study cohorts.ResultsPercentages of total iNKT and MAIT cells were dramatically dropped in blood, and reduced numbers of iNKT cells were observed in the sputum of COPD patients. Furthermore decreased DN and increased CD4+ iNKT subsets, while increased DN and decreased CD8+ MAIT subpopulations were measured in the blood of COPD patients. Reduced invariant TCR mRNA levels in COPD patients had confirmed these previous findings. The mRNA expression of CD1d and MR1 were increased in stable and exacerbating COPD patients; however both molecules were decreased upon antibiotic and systemic steroid treatments.ConclusionsOur results support the notion that both invariant T-cell populations are affected in COPD. Further detailed analysis of invariant T cells could shed more light into the complex interactions of these lymphocyte groups in COPD pathogenesis.

【 授权许可】

CC BY   
© The Author(s). 2017