Expression of pluripotency markers in the bovine uterus with adenomyosis

Reproductive Biology and Endocrinology

Research

Martyna Łupicka¹  Anna Korzekwa¹  Barbara Socha¹  Agata Szczepańska¹ 

[1] Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland;

关键词: Uterus;    Pluripotent cells;    Adenomyosis;    Cow;   

DOI  :  10.1186/s12958-015-0106-0

 received in 2015-06-18, accepted in 2015-09-17,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAdenomyosis is a proliferative uterine dysfunction with unknown aetiology. One possible mechanism of its development involves disturbances in stem cell differentiation in uterine tissue. Previously, we identified pluripotent/multipotent cells in the bovine uterus, therefore our present study focused on determining expression of pluripotency markers, NANOG, OCT4 and SOX2, in bovine adenomyotic tissues and cells.FindingsImmunolocalisation revealed protein expression of NANOG, OCT4 and SOX2 in both normal and adenomyotic uteri. mRNA expression for NANOG and OCT4 was increased in tissues obtained from uteri with adenomyosis compared to controls, but at the protein level there were no significant differences. mRNA expression for all three pluripotency markers was higher in myometrial cells isolated from uteri with adenomyotic lesions than in those isolated from normal uteri. The protein level of NANOG and SOX2 was decreased in stromal cells from adenomyotic tissues, whereas the level of OCT4 and SOX2 was increased in myometrial cells obtained from dysfunctional uteri.ConclusionsThe results indicate significant changes in expression of pluripotency markers in adenomyotic compared to normal uteri, which suggest the involvement of uterine stem cells in adenomyosis.

【 授权许可】

CC BY   
© Łupicka et al. 2015