Soybean and fish oil mixture increases IL-10, protects against DNA damage and decreases colonic inflammation in rats with dextran sulfate sodium (DSS) colitis

Lipids in Health and Disease

Research

Alessandra Gambero¹  Marcelo L Ribeiro¹  Vera LF Silveira²  Claudia MO Nascimento³  Karina V Barros³  Gilclay G Abreu³  Roberta AN Xavier³  Carlos AR Martinez⁴  Patrícia O Carvalho⁴ 

[1] Clinical Pharmacology and Gastroenterology Unit, São Francisco University Medical School, Bragança Paulista, SP, Brazil;Departamento de Ciências Biológicas, Universidade Federal de São Paulo, Campus Diadema, SP, Brazil;Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil;Multidisciplinary Research Unit, São Francisco University Medical School, Bragança Paulista, SP, Brazil;

关键词: Inflammatory Bowel Disease;    Ulcerative Colitis;    Corticosterone;    Comet Assay;    Dextran Sulfate Sodium;   

DOI  :  10.1186/1476-511X-9-68

 received in 2010-05-18, accepted in 2010-07-08,  发布年份 2010

来源: Springer

【 摘 要 】

It was investigated whether dietary polyunsaturated fatty acids (PUFA) could influence colonic injury, tissue DNA damage, cytokines and myeloperoxidase activity (MPO) and plasma corticosterone in DSS-induced colitis rats. Male weaning Wistar rats were fed for 47 days with an AIN-93 diet with control (C), fish (F) or a mixture of fish and soybean oil (SF). The colitis was induced from day 36 until day 42 by 3% DSS in drinking water. On day 48, blood samples were collected for corticosterone determination. The distal colon was excised for histological analysis and to quantify the cytokine (IL-4, IL-10 and INF-γ), MPO and DNA damage. The disease activity index (DAI) was recorded daily during colitis induction. The DAI, MPO, histological analyses showed decreases only in the SF group compared with the C group. IL-10 was increased and DNA damage was reduced in the groups F and SF, and an inverse correlation between these variables was found. There were no differences in corticosterone, IFN-γ and IL-4 levels. Soybean and fish oil mixture may be effective in improving colonic injury and DNA damage, and it could be an important complementary therapy in UC to reduce the use of anti-inflammatory drugs and prevent colorectal cancer.

【 授权许可】

Unknown   
© Barros et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.