Identification and differential regulation of microRNAs in response to methyl jasmonate treatment in Lycoris aurea by deep sequencing

BMC Genomics

Research Article

Sheng Xu¹  Ren Wang¹  Yilong Jiang¹  Xiaodan Li¹  Bing Xia¹  Yumei Jiang¹  Yikui Li¹  Zhengzhi Zhang²  Ning Wang³ 

[1] Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, 210014, Nanjing, China;National Center for Soybean Biotechnology and Division of Plant Sciences, University of Missouri, 65211, Columbia, MO, USA;National Center for Soybean Improvement/Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Nanjing Agricultural University, 210095, Nanjing, China;

关键词: Amaryllidaceae alkaloids;    Deep sequencing;    Degradome;    Lycoris aurea;    Methyl jasmonate;    MicroRNA;   

DOI  :  10.1186/s12864-016-2645-y

 received in 2015-04-03, accepted in 2016-04-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLycoris aurea is a medicine-valuable and ornamental herb widely distributed in China. Former studied have showed that methyl jasmonate (MJ) treatment could increase the content of glanthamine-a worldwide medicine for symptomatic treatment of Alzheimer’s disease in genus Lycoris plants. To explore the possible role of miRNAs in the regulation of jasmonic acid signaling pathway and uncover their potential correlations, we investigated the expression profiles of small RNAs (sRNAs) and their targets in Lycoris aurea, with MJ treatment by using next-generation deep sequencing.ResultsA total of 365 miRNAs were identified, comprising 342 known miRNAs (representing 60 miRNA families) and 23 novel miRNAs. Among them, 143 known and 11 novel miRNAs were expressed differently under MJ treatment. Quantitative real-time PCR of eight selected miRNAs validated the expression pattern of these loci in response to MJ treatment. In addition, degradome sequencing analysis showed that 32 target genes were validated to be targeted by the 49 miRNAs, respectively. Gene function and pathway analyses showed that these targets such as auxin response factors (ARFs), squamosa promoter-binding like (SPL) proteins, basic helix-loop-helix (bHLH) proteins, and ubiquitin-conjugating enzyme E2 are involved in different plant processes, indicating miRNAs mediated regulation might play important roles in L. aurea response to MJ treatment. Furthermore, several L. aurea miRNAs associated with their target genes that might be involved in Amaryllidaceae alkloids biosynthehsis were also analyzed.ConclusionsA number of miRNAs with diverse expression patterns, and complex relationships between expression of miRNAs and targets were identified. This study represents the first transcriptome-based analysis of miRNAs in Lycoris and will contribute to understanding the potential roles of miRNAs involved in regulation of MJ response.

【 授权许可】

CC BY   
© Xu et al. 2016