Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton

BMC Plant Biology

Research Article

Jinfa Zhang¹  Hengling Wei²  Shuli Fan²  Meizhen Song²  Man Wu²  Jiwen Yu²  Shuxun Yu³  Mingming Wei³ 

[1] Department of biology, New Mexico State University, 880033, Montreal, USA;State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, 455000, Anyang, Henan, P. R. China;State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, 455000, Anyang, Henan, P. R. China;College of Agronomy, Northwest A&F University, 712100, Yangling, Shaanxi, P. R. China;

关键词: Male Sterility;    Cytoplasmic Male Sterility;    Anther Development;    Degradome Sequence;    Genetic Male Sterility;   

DOI  :  10.1186/1471-2229-13-66

 received in 2012-11-19, accepted in 2013-04-06,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundGenetic male sterility (GMS) in cotton (Gossypium hirsutum) plays an important role in the utilization of hybrid vigor. However, the molecular mechanism of the GMS is still unclear. While numerous studies have demonstrated that microRNAs (miRNA) regulate flower and anther development, whether different small RNA regulations exist in GMS and its wild type is unclear. A deep sequencing approach was used to investigate the global expression and complexity of small RNAs during cotton anther development in this study.ResultsThree small RNA libraries were constructed from the anthers of three development stages each from fertile wild type (WT) and its GMS mutant cotton, resulting in nearly 80 million sequence reads. The total number of miRNAs and short interfering RNAs in the three WT libraries was significantly greater than that in the corresponding three mutant libraries. Sixteen conserved miRNA families were identified, four of which comprised the vast majority of the expressed miRNAs during anther development. In addition, six conserved miRNA families were significantly differentially expressed during anther development between the GMS mutant and its WT.ConclusionsThe present study is the first to deep sequence the small RNA population in G. hirsutum GMS mutant and its WT anthers. Our results reveal that the small RNA regulations in cotton GMS mutant anther development are distinct from those of the WT. Further results indicated that the differently expressed miRNAs regulated transcripts that were distinctly involved in anther development. Identification of a different set of miRNAs between the cotton GMS mutant and its WT will facilitate our understanding of the molecular mechanisms for male sterility.

【 授权许可】

Unknown   
© Wei et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.