Biological activities of extracts from Chenopodium ambrosioides Lineu and Kielmeyera neglecta Saddi

Annals of Clinical Microbiology and Antimicrobials

Research

Luiz Alberto Mattos Silva¹  Aline Oliveira da Conceição¹  Fernando Faustino de Oliveira²  Julianada Silva Santos³  Maria Helena Rossi³  Zulane Lima Sousa⁴  João Luciano Andrioli⁵ 

[1] Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Campus Prof. Soane Nazaré de Andrade, Km 16 – Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil;Departamento de Ciências Exatas e Tecnológicas, Universidade Estadual de Santa Cruz, Campus Prof. Soane Nazaré de Andrade, Km 16 – Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil;Instituto Biológico, Centro de Sanidade Animal, Av. Cons. Rodrigues Alves, 1252, 04014-002, São Paulo, Brazil;Programa de Pós-graduação em Biologia e Biotecnologia de Microrganismos, Universidade Estadual de Santa Cruz, Campus Prof. Soane Nazaré de Andrade, Km 16 – Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil;Programa de Pós-graduação em Biologia e Biotecnologia de Microrganismos, Universidade Estadual de Santa Cruz, Campus Prof. Soane Nazaré de Andrade, Km 16 – Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil;Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Campus Prof. Soane Nazaré de Andrade, Km 16 – Rodovia Ilhéus/Itabuna, 45662-900, Ilhéus, Brazil;

关键词: Antimicrobial;    Bioactivity;    Extract;    Chenopodium ambrosioides;    Kielmeyera neglecta;    Candida;   

DOI  :  10.1186/1476-0711-11-20

 received in 2012-05-22, accepted in 2012-07-22,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundChenopodium ambrosioides and Kielmeyera neglecta are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the detection of biologically active compounds.MethodsExtracts from these plants were extracted with hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and ethanol (EtOH) and assessed for their antimicrobial properties, bioactivity against Artemia salina Leach and antifungal action on the cell wall of Neurospora crassa.ResultsExtracts from C. ambrosioides (Hex, DCM and EtOH) and K. neglecta (EtOAc and EtOH) showed high bioactivity against A. salina (LD50 < 1000 μg/mL), which might be associated with cytotoxic activity against cancer cells. C. ambrosioides Hex and DCM showed specific activity against yeasts, highlighting the activity of hexanic extract against Candida krusei (MIC = 100 μg/mL). By comparing the inhibitory concentration of 50% growth (IC 50%) with the growth control, extracts from K. neglecta EtOAc and EtOH have shown activities against multidrug-resistant bacteria (Enterococcus faecalis ATCC 51299 and Staphylococcus aureus ATCC 43300), with IC 50% of 12.5 μg/mL The assay carried out on N. crassa allowed defining that extracts with antifungal activity do not have action through inhibition of cell wall synthesis.ConclusionsGenerally speaking, extracts from C. ambrosioides and K. neglecta showed biological activities that have made the search for bioactive substances in these plants more attractive, illustrating the success of their use in the Brazilian folk medicine.

【 授权许可】

Unknown   
© Sousa et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.