Lysophosphatidic acid plasma concentrations in healthy subjects: circadian rhythm and associations with demographic, anthropometric and biochemical parameters

Lipids in Health and Disease

Research

Anna Michalczyk¹  Krzysztof Safranow¹  Dariusz Chlubek¹  Marta Budkowska²  Barbara Dołęgowska³ 

[1] Department of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, 70-111, Szczecin, Poland;Department of Medical Analytics, Pomeranian Medical University in Szczecin, 70-111, Szczecin, Poland;Department of Microbiology, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, 70-111, Szczecin, Poland;

关键词: Lysophosphatidic acid;    Lysophospholipids;    Reference values;    Antropometric parameters;    Demographic parameters;    Circadian rhythm;    Healthy subjects;   

DOI  :  10.1186/s12944-017-0536-0

 received in 2017-06-07, accepted in 2017-07-13,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundLysophosphatidic acid (LPA) is a bioactive lipid with a wide biological activity. Previous studies have shown its potential usefulness as a diagnostic marker for ovarian cancer. The aim of the study was to investigate which factors may influence plasma LPA concentrations in healthy subjects and to propose reference values.MethodsThe study group consisted of 100 healthy subjects. From all of them the blood samples were taken at 7 a.m. (fasting state). From 40 volunteers additional blood samples were taken at 2 p.m., at 8 p.m. and at 2 a.m. next morning. Concentrations of LPA were measured in plasma samples using enzyme-linked immunosorbent assay.ResultsAnalysis of samples from 100 healthy volunteers showed significant influence of sex and age on plasma LPA. The reference range for the plasma LPA concentration corrected for age and sex, determined at 2.5–97.5 percentile interval is 0.14–1.64 μM. LPA correlates positively with BMI, serum total cholesterol, triacylglycerols, uric acid and negatively with estimated glomerular filtration rate and serum albumin. Concentration of LPA at 2 a.m. was lower than at 2 p.m. There were not any significant differences between plasma LPA at 7 a.m. and any other time of the day.ConclusionsPlasma LPA is associated with demographic, anthropometric and biochemical parameters. It seems that LPA concentrations have no specific circadian rhythm and the time of donation and fasting state have marginal effect on plasma LPA. These findings may be helpful in future incorporation of LPA as a diagnostic marker.

【 授权许可】

CC BY   
© The Author(s). 2017