A CLCA regulatory protein present in the chemosensory cilia of olfactory sensory neurons induces a Ca2+-activated Cl− current when transfected into HEK293

BMC Neuroscience

Research Article

Ricardo Delgado¹  Casilda V. Mura¹  María Graciela Delgado¹  Juan Bacigalupo¹  Diego Restrepo² 

[1] Department of Biology, Faculty of Sciences, University of Chile, Las Palmeras 3425, Ñuñoa, 7800024, Santiago, Chile;Department of Cell and Developmental Biology, Neuroscience Program and Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus, 80045, Aurora, CO, USA;

关键词: CLCA;    Ca-activated Cl current;    Anoctamin channels;    Olfactory cilia;   

DOI  :  10.1186/s12868-017-0379-7

 received in 2017-04-27, accepted in 2017-08-05,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCLCA is a family of metalloproteases that regulate Ca2+-activated Cl− fluxes in epithelial tissues. In HEK293 cells, CLCA1 promotes membrane expression of an endogenous Anoctamin 1 (ANO1, also termed TMEM16A)-dependent Ca2+-activated Cl− current. Motif architecture similarity with CLCA2, 3 and 4 suggested that they have similar functions. We previously detected the isoform CLCA4L in rat olfactory sensory neurons, where Anoctamin 2 is the principal chemotransduction Ca2+-activated Cl− channel. We explored the possibility that this protein plays a role in odor transduction.ResultsWe cloned and expressed CLCA4L from rat olfactory epithelium in HEK293 cells. In the transfected HEK293 cells we measured a Cl−-selective Ca2+-activated current, blocked by niflumic acid, not present in the non-transfected cells. Thus, CLCA4L mimics the CLCA1 current on its ability to induce the ANO1-dependent Ca2+-activated Cl− current endogenous to these cells. By immunocytochemistry, a CLCA protein, presumably CLCA4L, was detected in the cilia of olfactory sensory neurons co-expressing with ANO2.ConclusionThese findings suggests that a CLCA isoform, namely CLCA4L, expressed in OSN cilia, might have a regulatory function over the ANO2-dependent Ca2+-activated Cl− channel involved in odor transduction.

【 授权许可】

CC BY   
© The Author(s) 2017

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