Laboratory cohabitation challenge model for shrimp hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP)

BMC Veterinary Research

Research Article

Ornchuma Itsathitphaisarn¹  Pattana Jaroenlak¹  Siripong Thitamadee²  Kallaya Sritunyalucksana³  Paul Vinu Salachan⁴ 

[1] Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand;Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand;Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand;Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand;Shrimp–pathogen interaction (SPI) laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Yothi office, 10400, Bangkok, Thailand;Shrimp–pathogen interaction (SPI) laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Yothi office, 10400, Bangkok, Thailand;Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand;

关键词: Enterocytozoon hepatopenaei;    Shrimp microsporidian;    Cohabitation assay;    Penaeus vannamei;   

DOI  :  10.1186/s12917-016-0923-1

 received in 2016-08-26, accepted in 2016-12-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundEnterocytozoon hepatopenaei (EHP) causes hepatopancreatic microsporidiosis (HPM) in shrimp. It is probably endemic in Australasia and was first characterized and named from the giant or black tiger shrimp Penaeus monodon from Thailand in 2009. Later, it was also found to infect exotic Penaeus vannamei imported for cultivation in Asia. HPM is not normally associated with shrimp mortality, but information from shrimp farmers indicates that it is associated with significant growth retardation that is not clearly noticeable until 2–3 months of cultivation. In order to study modes of HPM transmission and to test possible control measures, a laboratory challenge model was needed that would mimic the mode of infection in shrimp ponds.ResultsWe describe successful transmission in a cohabitation model with natural E. hepatopenaei (EHP)-infected shrimp in closed, perforated plastic containers placed in aquaria together with free-swimming, uninfected shrimp. After a period of 14 days all the free-swimming shrimp tested positive by PCR (approximately 60% with heavy infections evident by 1-step PCR positive test results) and gave positive histological and in situ hybridization results for E. hepatopenaei (EHP) in the hepatopancreas.ConclusionsA laboratory cohabitation model for studying E. hepatopenaei (EHP) has been developed and used to confirm that E. hepatopenaei (EHP) can be directly transmitted horizontally among shrimp via water. The model will facilitate studies on methods to prevent the E. hepatopenaei (EHP) transmission.

【 授权许可】

CC BY   
© The Author(s). 2017