The EPIYA-ABCC motif pattern in CagA of Helicobacter pyloriis associated with peptic ulcer and gastric cancer in Mexican population

BMC Gastroenterology

Research Article

Tomás Manuel Poblete¹  Gloria Fernández-Tilapa¹  Fredy Omar Beltrán-Anaya¹  Adolfo Román-Román¹  Oscar Peralta-Zaragoza²  José de Sampedro³  Berenice Illades-Aguiar⁴  Miguel Ángel Rodríguez⁴  Oscar del Moral-Hernández⁴  Salomón Reyes⁵ 

[1] Clinical Research Laboratory, Academic Unit of Chemical-Biological Sciences, Autonomous University of Guerrero, Chilpancingo C.P., 39090, Guerrero, Mexico;Department of Chronic Infections and Cancer, Infectious Diseases Research Center, National Institute of Public Health, Av. Universidad No. 655, Cerrada los Pinos y Caminera, Colonia Santa María Ahuacatitlan, Cuernavaca C.P.Morelos, 62100, Mexico;General Hospital “Dr. Raymundo Abarca Alarcón”, Chilpancingo C.P., 39090, Guerrero, Mexico;Laboratory of Molecular Biomedicine, Academic Unit of Chemical-Biological Sciences, Autonomous University of Guerrero, Chilpancingo C.P., 39090, Guerrero, Mexico;State Institute of Oncology “Dr. Arturo Beltrán Ortega”, Acapulco C. P., 39570, Guerrero, Mexico;

关键词: cagA gene 3′ region;    CRPIA;    EPIYA;    CagA;    H. pylori;   

DOI  :  10.1186/s12876-014-0223-9

 received in 2014-08-13, accepted in 2014-12-17,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundHelicobacter pylori chronic infection is associated with chronic gastritis, peptic ulcer, and gastric cancer. Cytotoxin-associated gene A (cagA)-positive H. pylori strains increase the risk of gastric pathology. The carcinogenic potential of CagA is linked to its polymorphic EPIYA motif variants. The goals of this study were to investigate the frequency of cagA-positive Helicobacter pylori in Mexican patients with gastric pathologies and to assess the association of cagA EPIYA motif patterns with peptic ulcer and gastric cancer.MethodsA total of 499 patients were studied; of these, 402 had chronic gastritis, 77 had peptic ulcer, and 20 had gastric cancer. H. pylori DNA, cagA, and the EPIYA motifs were detected in total DNA from gastric biopsies by PCR. The type and number of EPIYA segments were determined by the electrophoretic patterns. To confirm the PCR results, 20 amplicons of the cagA 3′ variable region were sequenced, and analyzed in silico, and the amino acid sequence was predicted with MEGA software, version 5. The odds ratio (OR) was calculated to determine the associations between the EPIYA motif type and gastric pathology and between the number of EPIYA-C segments and peptic ulcers and gastric cancer.ResultsH. pylori DNA was found in 287 (57.5%) of the 499 patients, and 214 (74%) of these patients were cagA-positive. The frequency of cagA-positive H. pylori was 74.6% (164/220) in chronic gastritis patients, 73.6% (39/53) in peptic ulcer patients, and 78.6% (11/14) in gastric cancer patients. The EPIYA-ABC pattern was more frequently observed in chronic gastritis patients (79.3%, 130/164), while the EPIYA-ABCC sequence was more frequently observed in peptic ulcer (64.1%, 25/39) and gastric cancer patients (54.5%, 6/11). However, the risks of peptic ulcer (OR = 7.0, 95% CI = 3.3–15.1; p < 0.001) and gastric cancer (OR = 5.9, 95% CI = 1.5–22.1) were significantly increased in individuals who harbored the EPIYA-ABCC cagA gene pattern.ConclusionscagA-positive H. pylori is highly prevalent in southern Mexico, and all CagA variants were of the western type. The cagA alleles that code for EPIYA-ABCC motif patterns are associated with peptic ulcers and gastric cancer.

【 授权许可】

Unknown   
© Beltrán-Anaya et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.