Enhanced expression of long non-coding RNA NEAT1 is associated with the progression of gastric adenocarcinomas

World Journal of Surgical Oncology

Research

Jie Deng¹  Jianhai Sun¹  Fei Yan¹  Wujie Wei¹  Yanling Ma¹  Li Liu¹ 

[1] Department of Oncology of Zhongshan Hospital, Wuhan University, No. 26 Zhongshan Road, 430033, Wuhan, Hubei, China;

关键词: Gastric adenocarcinoma;    Long non-coding RNA;    NEAT1;    Cell proliferation;   

DOI  :  10.1186/s12957-016-0799-3

 received in 2015-11-07, accepted in 2016-02-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLong non-coding RNAs (lncRNAs) are emerging as new players in the cancer. The aim of this study was to examine the abnormalities of NEAT1 (nuclear paraspeckle assembly transcript 1, also known as MENε/β) in gastric adenocarcinomas (GACs).MethodsOne hundred thirty-one GAC tissues and matched adjacent normal tissues (ANTs) were collected from patients who undergone surgery. Differences in of NEAT1 expression were examined via quantitative reverse transcriptase PCR (qRT-PCR). WST-1 assay and transwell assay were carried out in vitro to investigate the proliferation and migration of GAC cells with alteration in NEAT1 long non-coding RNA (lncRNA) expression.ResultsThe expression levels of lncRNA NEAT1 were significantly elevated in GAC tissues (P < 0.001) compared with ANTs. There was also a statistical difference in NEAT1 expression between early and advanced GACs (P = 0.0111). GACs with lymph node metastasis (LNM) expressed higher levels of NEAT1 lncRNA compared with those without LNM (P = 0.004). In the in vitro experiments, the proliferation but not migration of GAC cells was attenuated after NEAT1 knockdown by RNA interference.ConclusionsExpression of NEAT1 lncRNA was enhanced in GACs; and NEAT1 may influence GAC progression by promoting tumor growth.

【 授权许可】

CC BY   
© Ma et al. 2016