In vitro expansion of the mammary stem/progenitor cell population by xanthosine treatment

BMC Cell Biology

Research Article

Ratan K Choudhary¹  Anthony V Capuco² 

[1] Department of Animal and Avian Sciences, University of Maryland, College Park, MD, USA;Department of Animal and Avian Sciences, University of Maryland, College Park, MD, USA;Bovine Functional Genomics Laboratory, USDA-ARS, Beltsville, MD, USA;

关键词: Mammary stem cell;    Self renewal;    Symmetric division;    FNDC3B;    Telomerase;    Bovine;   

DOI  :  10.1186/1471-2121-13-14

 received in 2012-01-12, accepted in 2012-06-14,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMammary stem cells are critical for growth and maintenance of the mammary gland and therefore are of considerable interest for improving productivity and efficiency of dairy animals. Xanthosine treatment has been demonstrated to promote expansion of putative mammary stem cells in vivo, and hepatic and hair follicle stem cells in vitro. In the latter, xanthosine promoted the symmetrical division of hepatic and hair follicle stem cells. The objective of this study was to determine if treating primary cultures of bovine mammary epithelial cells (MEC) with xanthosine increases the stem/progenitor cell population by promoting symmetrical division of mammary stem cells.ResultsIn vitro treatment with xanthosine increased the population of MEC during the exponential phase of cell growth, reducing the doubling time from 86 h in control cultures to 60 h in xanthosine-treated cultures. The bromodeoxyuridine (BrdU) labeling index and the proportion of MEC in S-phase both were increased by xanthosine treatment, indicating that increased cell accretion was due to increased cell proliferation. Analysis of daughter-pairs indicated that xanthosine promoted a shift from asymmetric to symmetric cell division. Moreover, the 30 % increase in symmetric cell division was concomitant with an increase in the proportion of MEC that were positive for a putative stem cell marker (FNDC3B) and a trend toward increased telomerase activity. These results suggest that xanthosine treatment in vitro can increase cell proliferation, promote symmetric cell division and enhance stem/progenitor cell activity.ConclusionsXanthosine treatment increased the proliferation rate of bovine MEC in vitro. This was likely to be mediated by an increase in the proportion of stem/progenitor cells in the MEC population due to promotion of symmetrical stem cell division by xanthosine.

【 授权许可】

CC BY   
© Choudhary and Capuco; licensee BioMed Central Ltd. 2012