Effect of carbon black nanomaterial on biological membranes revealed by shape of human erythrocytes, platelets and phospholipid vesicles

Journal of Nanobiotechnology

Research

Henry Hägerstrand¹  Veno Kononenko²  Barbara Drašler²  Damjana Drobne²  Darko Makovec³  Manca Pajnič⁴  Judita Lea Krek⁴  Veronika Kralj-Iglič⁴  Roman Štukelj⁴  Metka Šimundić⁴  Vid Šuštar⁵ 

[1] Department of Biosciences, BioCity, Åbo Akademi University, BioCity, Artillerigatan 6, SF-20520, Åbo/Turku, Finland;Group of Nanobiology and Nanotoxicology, University of Ljubljana, Biotechnical Faculty, Večna pot 111, SI-1000, Ljubljana, Slovenia;J. Stefan Institute, Jamova 39, SI-1000, Ljubljana, Slovenia;Laboratory of Clinical Biophysics, University of Ljubljana, Faculty of Health Sciences, Zdravstvena pot 5, SI-1000, Ljubljana, Slovenia;Lymphocyte Cytoskeleton Group, Institute of Biomedicine/Pathology, BioCity, University of Turku, Tykistökatu 6B, SF-20520, Turku, Finland;

关键词: Carbon black nanomaterial;    Carbon black;    Air pollution;    Nanotoxicity;    Cell membranes;    Phospholipid bilayer;    Model systems;    Blood cells;   

DOI  :  10.1186/s12951-015-0087-3

 received in 2014-12-03, accepted in 2015-03-16,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundWe studied the effect of carbon black (CB) agglomerated nanomaterial on biological membranes as revealed by shapes of human erythrocytes, platelets and giant phospholipid vesicles. Diluted human blood was incubated with CB nanomaterial and observed by different microscopic techniques. Giant unilamellar phospholipid vesicles (GUVs) created by electroformation were incubated with CB nanomaterial and observed by optical microscopy. Populations of erythrocytes and GUVs were analyzed: the effect of CB nanomaterial was assessed by the average number and distribution of erythrocyte shape types (discocytes, echinocytes, stomatocytes) and of vesicles in test suspensions, with respect to control suspensions. Ensembles of representative images were created and analyzed using computer aided image processing and statistical methods. In a population study, blood of 14 healthy human donors was incubated with CB nanomaterial. Blood cell parameters (concentration of different cell types, their volumes and distributions) were assessed.ResultsWe found that CB nanomaterial formed micrometer-sized agglomerates in citrated and phosphate buffered saline, in diluted blood and in blood plasma. These agglomerates interacted with erythrocyte membranes but did not affect erythrocyte shape locally or globally. CB nanomaterial agglomerates were found to mediate attractive interaction between blood cells and to present seeds for formation of agglomerate - blood cells complexes. Distortion of disc shape of resting platelets due to incubation with CB nanomaterial was not observed. CB nanomaterial induced bursting of GUVs while the shape of the remaining vesicles was on the average more elongated than in control suspension, indicating indirect osmotic effects of CB nanomaterial.ConclusionsCB nanomaterial interacts with membranes of blood cells but does not have a direct effect on local or global membrane shape in physiological in vitro conditions. Blood cells and GUVs are convenient and ethically acceptable methods for the study of effects of various substances on biological membranes and therefrom derived effects on organisms.

【 授权许可】

Unknown   
© Pajnič et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.