Gold nanoparticles as multimodality imaging agents for brain gliomas

Journal of Nanobiotechnology

Research

Dueng-Yuan Hueng¹  Sheng-Feng Lai²  Bai-Hung Ko³  Y. Hwu⁴  Ann Chen⁵  Hsiang-Hsin Chen⁶  Cyril Petibois⁶  Shuk-Man Ka⁷  Chia-Chi Chien⁸  Chia-Ju Chang⁸  Shun-Ming Yang⁸  G. Margaritondo⁹ 

[1] Department of Biochemistry, School of Medicine, National Defense Medical Center, 114, Taipei, Taiwan;Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, 114, Taipei, Taiwan;Department of Engineering Science, National Cheng Kung University, 701, Tainan, Taiwan;Department of Engineering Science, National Cheng Kung University, 701, Tainan, Taiwan;Institute of Physics, Academia Sinica, Nankang, 115, Taipei, Taiwan;Department of Engineering Science, National Cheng Kung University, 701, Tainan, Taiwan;Institute of Physics, Academia Sinica, Nankang, 115, Taipei, Taiwan;Advanced Optoelectronic Technology Center, National Cheng Kung University, 701, Tainan, Taiwan;Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, 114, Taipei, Taiwan;Inserm U1029 LMMA, University of Bordeaux, 33600, Pessac Cedex, France;Institute of Aerospace and Undersea Medicine, School of Medicine, National Defense Medical Center, 114, Taipei, Taiwan;Institute of Physics, Academia Sinica, Nankang, 115, Taipei, Taiwan;School of Basic Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland;

关键词: Glioma Cell;    Advanced Photon Source;    Orthotopic Xenografting;    Single Photon Emission Computer Tomography;    CdWO4;   

DOI  :  10.1186/s12951-015-0140-2

 received in 2015-06-26, accepted in 2015-10-22,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundNanoparticles can be used for targeted drug delivery, in particular for brain cancer therapy. However, this requires a detailed analysis of nanoparticles from the associated microvasculature to the tumor, not easy because of the required high spatial resolution. The objective of this study is to demonstrate an experimental solution of this problem, based in vivo and post-mortem whole organ imaging plus nanoscale 3-dimensional (3D) X-ray microscopy.ResultsThe use of gold nanoparticles (AuNPs) as contrast agents paved the way to a detailed high-resolution three dimensional (3D) X-ray and fluorescence imaging analysis of the relation between xenografted glioma cells and the tumor-induced angiogenic microvasculature. The images of the angiogenic microvessels revealed nanoparticle leakage. Complementary tests showed that after endocytotic internalization fluorescent AuNPs allow the visible-light detection of cells.ConclusionsAuNP-loading of cells could be extended from the case presented here to other imaging techniques. In our study, they enabled us to (1) identify primary glioma cells at inoculation sites in mice brains; (2) follow the subsequent development of gliomas. (3) Detect the full details of the tumor-related microvasculature; (4) Finding leakage of AuNPs from the tumor-related vasculature, in contrast to no leakage from normal vasculature.

【 授权许可】

CC BY   
© Lai et al. 2015