Characterization of two Lactococcus lactis zinc membrane proteins, Llmg_0524 and Llmg_0526, and role of Llmg_0524 in cell wall integrity

BMC Microbiology

Research Article

Bénédicte Cesselin¹  Célia Roussel¹  Philippe Gaudu²  Rémy Cachon³ 

[1] INRA, UMR1319 Micalis, F-78350, Jouy-en-Josas, France;AgroParisTech, UMR Micalis, F-78350, Jouy-en-Josas, France;INRA, UMR1319 Micalis, F-78350, Jouy-en-Josas, France;AgroParisTech, UMR Micalis, F-78350, Jouy-en-Josas, France;Institut Micalis UMR1319 et AgroParisTech, Domaine de Vilvert, 78352 Jouy-en-Josas, Cedex, France;UMR A 02.102 Unité Procédés Alimentaires et Microbiologiques, AgroSup Dijon-Université de Bourgogne, 1-esplanade Erasme, F-21000, Dijon, France;

关键词: Cysteine;    Zinc;    Membrane proteins;    Growth;    Cumene hydroperoxide;    Lysozyme;   

DOI  :  10.1186/s12866-015-0587-1

 received in 2015-03-26, accepted in 2015-10-23,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundDue to its extraordinary chemical properties, the cysteine amino acid residue is often involved in protein folding, electron driving, sensing stress, and binding metals such as iron or zinc. Lactococcus lactis, a Gram-positive bacterium, houses around one hundred cysteine-rich proteins (with the CX2C motif) in the cytoplasm, but only a few in the membrane.ResultsIn order to understand the role played by this motif we focused our work on two membrane proteins of unknown function: Llmg_0524 and Llmg_0526. Each of these proteins has two CX2C motifs separated by ten amino-acid residues (CX2CX10CX2C). Together with a short intervening gene (llmg_0525), the genes of these two proteins form an operon, which is induced only during the early log growth phase. In both proteins, we found that the CX2CX10CX2C motif chelated a zinc ion via its cysteine residues, but the sphere of coordination was remarkably different in each case. In the case of Llmg_0524, two of the four cysteines were ligands of a zinc ion whereas in Llmg_0526, all four residues were involved in binding zinc. In both proteins, the cysteine-zinc complex was very stable at 37 °C or in the presence of oxidative agents, suggesting a probable role in protein stability. We found that the complete deletion of llmg_0524 increased the sensitivity of the mutant to cumene hydroperoxide whereas the deletion of the cysteine motif in Llmg_0524 resulted in a growth defect. The latter mutant was much more resistant to lysozyme than other strains.ConclusionsOur data suggest that the CX2CX10CX2C motif is used to chelate a zinc ion but we cannot predict the number of cysteine residue involved as ligand of metal. Although no other motif is present in sequence to identify roles played by these proteins, our results indicate that Llmg_0524 contributes to the cell wall integrity.

【 授权许可】

CC BY   
© Roussel et al. 2015